Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
yCCE1

Western result: +/-

GST GST-yCCE1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: yCCE1
CBI Antibody Core Code: A0312
"Lot" Number: 2 Position: 100(160-259)
% Identical Homology of Target to Mouse Protein: 61% (NP_061249.1| phosphodiesterase 3A, cGMP inhibited; cGMP-inhibited phosphodiesterase)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PILKVNILEQILFSNLENKMKYTNKIPNTSKLRYMVCSSD
PHRMTSYWCIPREETPTSSKKLKSNKHSKDSRIKLVKKIL
STSILEGNSTSSTKLVEFIG

Sequence Name: yCCE1
Full name: ORF YKL011c
UniGene Number: N.A.
Accession Number: CAA81846
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 353
NCBI information page: click here

Full length sequence:
MSTAQKAKILQLIDSCCQNAKSTQLKSLSFVIGAVNGTTKEAKRTYIQEQ
CEFLEKLRQQKIREGRINILSMDAGVSNFAFSKMQLLNNDPLPKVLDWQK
INLEEKFFQNLKKLSLNPAETSELVFNLTEYLFESMPIPDMFTIERQRTR
TMSSRHILDPILKVNILEQILFSNLENKMKYTNKIPNTSKLRYMVCSSDP
HRMTSYWCIPREETPTSSKKLKSNKHSKDSRIKLVKKILSTSILEGNSTS
STKLVEFIGVWNNRIRNALTKKKSFKLCDILEIQDNSGVRKDDDLADSFL
HCLSWMEWLKNYESITELLNSKTLVKTQFGQVFEFCENKVQKLKFLQNTY
NND