Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Rad7

Western result: +

GST GST-yRad7 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: yRad7
CBI Antibody Core Code: A1065a
"Lot" Number: 1 Position: 100 (35-134)
% Identical Homology of Target to Mouse Protein: 31% (NP_064346.2| kinesin family member 21B; N-5 kinesin)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
ENIKQKWYQRQSKKQEDATDEKKGKAEDDSFTAEISRVVE
DEEIDEIGTGSGTETERAQVSYDARMKLVPADSDEEEYET
SHISDTPVSLSSANDRESLT

Sequence Name: Rad7
Full name:
UniGene Number: N.A.
Accession Number: YJR052W
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 565
NCBI information page:

Full length sequence:
MYRSRNRPKRGGENEVKGPNSALTQFLREEGISAENIKQKWYQRQSKKQE
DATDEKKGKAEDDSFTAEISRVVEDEEIDEIGTGSGTETERAQVSYDARM
KLVPADSDEEEYETSHISDTPVSLSSANDRESLTKKRQNTAKIIQNRRRK
RKRAADLLDRRVNKVSSLQSLCITKISENISKWQKEADESSKLVFNKLRD
VLGGVSTANLNNLAKALSKNRALNDHTLQLFLKTDLKRLTFSDCSKISFD
GYKTLAIFSPHLTELSLQMCGQLNHESLLYIAEKLPNLKSLNLDGPFLIN
EDTWEKFFVIMKGRLEEFHISNTHRFTDKSLSNLLINCGSTLVSLGLSRL
DSISNYALLPQYLVNDEFHSLCIEYPFNEEDVNDEIIINLLGQIGRTLRK
LVLNGCIDLTDSMIINGLTAFIPEKCPLEVLSLEESDQITTDSLSYFFSK
VELNNLIECSFRRCLQLGDMAIIELLLNGARDSLRSLNLNSLKELTKEAF
VALACPNLTYLDLGFVRCVDDSVIQMLGEQNPNLTVIDVFGDNLVTEKAT
MRPGLTLIGRQSDSI