Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
KSS1

Western result: +

control KSS1 A1061b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: KSS1
CBI Antibody Core Code: A1061b
"Lot" Number: 1 Position: 20 (315-349)
% Identical Homology of Target to Mouse Protein: 75% (NP_035291.3| mitogen-activated protein kinase 11; protein kinase, mitogen activated kinase, 11, p38beta)
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
MYHDPSDEPEYPPLNLDDEF

Antibody Name/Abbreviation: KSS1
CBI Antibody Core Code: A1061a
"Lot" Number: 2 Position: 100 (250-349)
% Identical Homology of Target to Mouse Protein: 52%
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
FNQIKSKRAKEYIANLPMRPPLPWETVWSKTDLNPDMIDL
LDKMLQFNPDKRISAAEALRHPYLAMYHDPSDEPEYPPLN
LDDEFWKLDNKIMRPEEEEE

Sequence Name: KSS1
Full name:
UniGene Number: N.A.
Accession Number: P14681
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 368
NCBI information page:

Full length sequence:
MARTITFDIPSQYKLVDLIGEGAYGTVCSAIHKPSGIKVAIKKIQPFSKK
LFVTRTIREIKLLRYFHEHENIISILDKVRPVSIDKLNAVYLVEELMETD
LQKVINNQNSGFSTLSDDHVQYFTYQILRALKSIHSAQVIHRDIKPSNLL
LNSNCDLKVCDFGLARCLASSSDSRETLVGFMTEYVATRWYRAPEIMLTF
QEYTTAMDIWSCGCILAEMVSGKPLFPGRDYHHQLWLILEVLGTPSFEDF
NQIKSKRAKEYIANLPMRPPLPWETVWSKTDLNPDMIDLLDKMLQFNPDK
RISAAEALRHPYLAMYHDPSDEPEYPPLNLDDEFWKLDNKIMRPEEEEEV
PIEMLKDMLYDELMKTME