Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
MSG5

Antibody Name/Abbreviation: MSG5
CBI Antibody Core Code: A1057b
"Lot" Number: 1 Position: 20 (15-34)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
DIDFKPNSPRSLQNRNTKNL

Antibody Name/Abbreviation: MSG5
CBI Antibody Core Code: A1057a
"Lot" Number: 2 Position: 100 (119-218)
% Identical Homology of Target to Mouse Protein: 36% (NP_080684.1| synuclein, alpha interacting protein (synphilin); synphilin-1)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
YTKSSTVSSISKLSSSSPLSSFSEKPHLNRVHSLSVKTKD
LKLKGIRGRSQTISGLETSTPISSTREGTLDSTDVNRFSN
QKNMQTTLIFPEEDSDLNID

Other western:
Code: A1057a
Lot: 1

Western result: +

GST GST-MSG5 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: MSG5
Full name:
UniGene Number: N.A.
Accession Number: P38590
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 489
NCBI information page:

Full length sequence:
MQFHSDKQHLDSKTDIDFKPNSPRSLQNRNTKNLSLDIAALHPLMEFSSP
SQDVPGSVKFPSPTPLNLFMKPKPIVLEKCPPKVSPRPTPPSLSMRRSEA
SIYTLPTSLKNRTVSPSVYTKSSTVSSISKLSSSSPLSSFSEKPHLNRVH
SLSVKTKDLKLKGIRGRSQTISGLETSTPISSTREGTLDSTDVNRFSNQK
NMQTTLIFPEEDSDLNIDMVHAEIYQRTVYLDGPLLVLPPNLYLYSEPKL
EDILSFDLVINVAKEIPNLEFLIPPEMAHKIKYYHIEWTHTSKIVKDLSR
LTRIIHTAHSQGKKILVHCQCGVSRSASLIVAYIMRYYGLSLNDAYNKLK
GVAKDISPNMGLIFQLMEWGTMLSKNSPGEEGETVHMPEEDDIGNNEVSS
TTKSYSSASFRSFPMVTNLSSSPNDSSVNSSEVTPRTPATLTGARTALAT
ERGEDDEHCKSLSQPADSLEASVDNESISTAPEQMMFLP