Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
DIG1

Western result: +

control DIG1 A1055b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: DIG1
CBI Antibody Core Code: A1055b
"Lot" Number: 1 Position: 20 (381-400)
% Identical Homology of Target to Mouse Protein: N.A.
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
TFTTFQRTSQPQQQSPSLLQ

Antibody Name/Abbreviation: DIG1
CBI Antibody Core Code: A1055a
"Lot" Number: 2 Position: 100 (5-104)
% Identical Homology of Target to Mouse Protein: 26% (NP_033007.1| protein tyrosine phosphatase, receptor type, G)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
ARLRTTAEDTSIAKSTQDPIGDTEISVANAKGSSDSNIKN
SPGGNSVGQESELEHVPEEDDSGDKEADHEDSETATAKKR
KAQPLKNPKKSLKRGRVPAP

Sequence Name: DIG1
Full name:
UniGene Number: N.A.
Accession Number: Q03063
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 452
NCBI information page:

Full length sequence:
MAVSARLRTTAEDTSIAKSTQDPIGDTEISVANAKGSSDSNIKNSPGGNS
VGQESELEHVPEEDDSGDKEADHEDSETATAKKRKAQPLKNPKKSLKRGR
VPAPLNLSDSNTNTHGGNIKDGNLASSNSAHFPPVANQNVKSAPAQVTQH
SKFQPRVQYLGKASSRQSIQVNNSSNSYGKPHMPSAGIMSAMNPYMPMNR
YIMSPYYNPYGIPPPHMLNKPIMTPYVSYPYPMGPRTSIPYAMQGGNARP
YEENEYSASNYRNKRVNDSYDSPLSGTASTGKTRRSEEGSRNSSVGSSAN
AGPTQQRADLRPADMIPAEEYHFERDALLSANTKARSASTSTSTSTSTNR
DRSSWHEAEPNKDEEEGTDLAIEDGAVPTPTFTTFQRTSQPQQQSPSLLQ
GEIRLSSHIFAFEFPLSSSNVDKKMFMSICNKVWNESKELTKKSSSHHRT
GK