Norway rat (Rat) ( Rattus norvegicus )
PLCb1-C

Western result: +

GST GST-PLCb1-N (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PLCb1-N
CBI Antibody Core Code: A0929
"Lot" Number: 1 Position: 20(410-509)
% Identical Homology of Target to Mouse Protein: 70% (NP_062651.1| phospholipase C, beta 1; phospholipase C-beta-1a)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
SFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESG
VPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNT
YSDSSSVFEPSSPGAGEADT

Sequence Name: PLCb1-C
Full name: phospholipase C-beta1
UniGene Number: N.A.
Accession Number: P10687
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 1216
NCBI information page:

Full length sequence:
magaqpgvhalqlkpvcvsdslkkgtkfvkwdddstivtpiilrtdpqgf
ffywtdqnketelldlslvkdarcgkhakapkdpklrelldvgnighleq
rmitvvygpdlvnishlnlvafqeevakewtnevfslatnllaqnmsrda
flekaytklklqvtpegriplkniyrlfsadrkrvetaleacslpssrnd
sipqedftpdvyrvflnnlcprpeidnifsefgakskpyltvdqmmdfin
lkqrdprlneilypplkqeqvqvliekyepnsslakkgqmsvdgfmryls
geengvvspekldlnedmsqplshyfinsshntyltagqlagnssvemyr
qvllsgcrcveldcwkgrtaeeepvithgftmtteisfkevieaiaecaf
ktspfpillsfenhvdspkqqakmaeycrlifgdallmeplekyplesgv
plpspmdlmykilvknkkkshkssegsgkkklseqasntysdsssvfeps
spgageadtesddddddddckkssmdegtagseamateemsnlvnyiqpv
kfesfetskkrnksfemssfvetkgleqltkspvefveynkmqlsriypk
gtrvdssnympqlfwnagcqmvalnfqtvdlamqinmgmyeyngksgyrl
kpefmrrpdkhfdpftegivdgivantlsvkiisgqflsdkkvgtyvevd
mfglpvdtrrkafktktsqgnavnpvweeepivfkkvvlpslaclriaay
eeggkfighrilpvqairpgyhyiclrnernqplmlpavfvyievkdyvp
dtyadviealsnpiryvnlmeqrakqlaaltledeeevkkeadpgetsse
apsetrttpaengvnhtatlapkppsqaphsqpapgsvkapaktedliqs
vlteveaqtieelkqqksfvklqkkhykemkdlvkrhhkkttelikehtt
kyneiqndylrrraaleksakkdskkksepsspdhgssaieqdlaaldae
mtqklidlkdkqqqqllnlrqeqy