Norway rat (Rat) ( Rattus norvegicus )
mWNK1

Western result: +/-, 2 bands

GST GST-mWNK1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: mWNK1
CBI Antibody Core Code: A0346
"Lot" Number: 1 Position: 100(801-900)
% Identical Homology of Target to Mouse Protein: 87% (NP_941992.1| protein kinase, lysine deficient 1)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
QSQPTQPVPLVSSVDSAHSDVASGMSDGNENAPSSSGRHE
GRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVE
CQLETHNRKMVTFKFDLDGD

Sequence Name: mWNK1
Full name: Protein kinase, lysine deficient 1 (Prkwnk1)
UniGene Number: N.A.
Accession Number: NM_053794
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 2126
NCBI information page: click here

Full length sequence:
MSDGTAEKQSGTPGFLSPPAPVPKNGSSSDSSVGEKLGAAVADSGIGRTE
EYRRRRHTMDKDSRGAAATTTPTEHRFFRRSVICDSNATALELPGLPLSI
PQPSVPAVVPQSAPPEPHREETLTATVASQVSQQPSAAASPGEQAVVGSA
TATVPSSTSKDRPVSQPSLVGSKEEPPPSRSGSGSGGASAKEPQEERNQQ
QDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWC
ELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLV
TELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDL
KCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYD
ESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIP
EVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAI
KLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGD
HKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEESSFKQQNEQQASVS
QAGIQPLSVASTGIPTAPTTSASVSTQVEPEEPEADQHQQLQYQQPSISV
LSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSIGTAPGHTVSSIQ
AQSQPHGVYPPSSMAQGQNQGQPSSSLAGVLSSQPVQHPQQQGIQPTVPP
QQAVQYSLPQAASSSEGTVQPVSQPQVSAGTQSSTQGVSQAAPPEQTPIT
QSQPTQPVPLVSSVDSAHSDVASGMSDGNENAPSSSGRHEGRTTKRHYRK
SVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGD
NPEEIATIMVNNDFILAIERESFVAQVREIIEKADEMLSEDVSVEPEGDQ
GLESLQGKDDYGFPGSQKLEGEFKQPIAVSSMPQQIGVPTSSLTQVVHSA
GRRFIVSPVPESRLRESKIFTSEIPDPVAASTSQGPGMNLSHSASSLSLQ
QAFSELKHGQMTEGPNTAPPNFNHPGPTFSPFLTSIAGVQTVAASTPSVS
VPITSSPLNDISTSVMQSEGALPTDKGIGGVTTSTGVVASGGLTTLSVSE
TPTLSSAVSSSTAPAVVTVSTTSQPVQAFTSGSIASSTGSFPSGTFSTTT
GTTVSSVAVPNAKPPTVLLQQVAGNTAGVAIVTSVSTTTPFPAMASQPSL
PLGSSTSAPTLAETVVVSAHSLDKASHSSTAGLGLSFCAPSSSSSSGTAV
SSSVSQPGIVHPLVISSAIASTPVLPQPAVPTSTPLLPQVPNIPPLVQPV
ANVPAVQQTLIHSQPQPALLPNQPHTHCPEMDADTQSKAPGIDDIKTLEE
KLRSLFSEHSSSGTQHASVSLETPLVVETVTPGIPTTAVAPSKLMTSTTS
TCLPPTNLPLGTAGMPVMPVGTPGQVSTPGTHASAPASTATGAKPGTTPP
KPSLTKTVVPPVGTELSAGTVPCEQLPPFPGPSLIQTQQPLEDLDAQLRR
TLSPETIPVTPAVGPLSTMSSTAVTEAGSQPQKDGTEVHVTASSSGAGVV
KMGRFQVSVTMDDAQKERKNRSEDTKSVHFESSTSESSVLSSSSPESTLV
KPEPNGITVSGISLDVPDSTHRTPTPEAKSETGQPTKVGRFQVTTTANKV
GRFSVSRTEDKVTELKKEGPVTSPFRDSEQTVIPAAIPKKEKPELAEPSH
LNGPSSDLEAAFLSRGGEDGSGSPHSPPHLCSKSLPIQTLSQSLSNSFNS
SYMSSDNESDIEDEDLRLELRRLREKHLKEIQDLQSRQKHEIESLYTKLG
KVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQS
GTSVLNPQQTLHPPGNTPETGHNQLLQPLKPSPSSDNLYSAFTSDGAISI
PSLSAPGQGTSSTNTVGGTVSSQAAQAQPPAMTSSRKGTFTDDLHKLVDN
WARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNMGGSTPIS
AASATSLGHFTKSMCPPQQYGFPAAPFGTQWSGTGGPAPQPLGQFQPVGT
TSLQNFNISNLQKSISNPPSSNLRTT