Norway rat (Rat) ( Rattus norvegicus )
TrkA

Western result: +

GST GST-TrkA (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TrkA
CBI Antibody Core Code: A0729
"Lot" Number: 2 Position: 100(112-211)
% Identical Homology of Target to Mouse Protein: 75% (XP_283871.1| neurotrophic tyrosine kinase, receptor, type 1)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
HFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLH
CSCALLWLQRWEQEDLCGVYTQKLQGSGSGDQFLPLGHNN
SCGVPSVKIQMPNDSVEVGD

Sequence Name: TrkA
Full name: High affinity nerve growth factor receptor precursor (p140-TrkA) (Slow nerve growth factor receptor) (Trk-A)
UniGene Number: N.A.
Accession Number: P35739
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 799
NCBI information page: click here

Full length sequence:
MLRGQRHGQLGWHRPAAGLGGLVTSLMLACACAASCRETCCPVGPSGLRC
TRAGTLNTLRGLRGAGNLTELYVENQRDLQRLEFEDLQGLGELRSLTIVK
SGLRFVAPDAFHFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPL
HCSCALLWLQRWEQEDLCGVYTQKLQGSGSGDQFLPLGHNNSCGVPSVKI
QMPNDSVEVGDDVFLQCQVEGQALQQADWILTELEGTATMKKSGDLPSLG
LTLVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGKAVEQHH
WCIPFSVDGQPAPSLRWFFNGSVLNETSFIFTQFLESALTNETMRHGCLR
LNQPTHVNNGNYTLLAANPYGQAAASIMAAFMDNPFEFNPEDPIPVSFSP
VDTNSTSRDPVEKKDETPFGVSVAVGLAVSAALFLSALLLVLNKCGQRSK
FGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTEGKGSGLQGHIMENPQYF
SDTCVHHIKRQDIILKWELGEGAFGKVFLAECYNLLNDQDKMLVAVKALK
ETSENARQDFHREAELLTMLQHQHIVRFFGVCTEGGPLLMVFEYMRHGDL
NRFLRSHGPDAKLLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLASLHFV
HRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDYYRVGGRTMLPIRWMPPE
SILYRKFSTESDVWSFGVVLWEIFTYGKQPWYQLSNTEAIECITQGRELE
RPRACPPDVYAIMRGCWQREPQQRLSMKDVHARLQALAQAPPSYLDVLG