Norway rat (Rat) ( Rattus norvegicus )
P35-C

Western result: +

control P35-C (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: P35-C
CBI Antibody Core Code: A0506
"Lot" Number: 1 Position: 12(293-304)
% Identical Homology of Target to Mouse Protein: 100% (NP_034001.1| cyclin-dependent kinase 5, regulatory subunit (p35))
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
SGQEDKKRLLLG

Sequence Name: P35-C
Full name: cyclin-dependent kinase 5, regulatory subunit 1
UniGene Number: N.A.
Accession Number: NP_446343
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 307
NCBI information page: click here

Full length sequence:
MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVL
PWKRIVAVSAKKKNSKKAQPNSSYQSNIAHLNNENLKKSLSCANLSTFAQ
PPPAQPPAPPASQLSGSQTGVSSSVKKAPHPAITSAGTPKRVIVQASTSE
LLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPAN
VVFLYMLCRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLV
ESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESGQEDKKR
LLLGLDR