Norway rat (Rat) ( Rattus norvegicus )
rNADPHOb

Western result: ++

GST GST-rNADPHOb (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: rNADPHOb
CBI Antibody Core Code: A0365
"Lot" Number: 1 Position: 100(471-570)
% Identical Homology of Target to Mouse Protein: 97% (NP_031833.2| cytochrome b-245, beta polypeptide)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
ANFLSYNIYLTGWDESQANHFAVHHDEEKDVITGLKQKTL
YGRPNWDNEFKTIASQHPNTRIGVFLCGPEALAKTLSKQS
ISNSESGPRGVHFIFNKENF

Sequence Name: rNADPHOb
Full name: NADPH oxidase beta subunit gp91phox (gp91phox)
UniGene Number: N.A.
Accession Number: AF298656
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 570
NCBI information page: click here

Full length sequence:
MGNWAVNEGLSIFVILVWLGLNVFLFVKYYKVYDDEPKYNYTRKLLGSAL
ALARAPAACLNFNCMLILLPVCRNLLSFLRGSSACCSTRIRRQLDRNLTF
HKMVAWMIALHTAIHTIAHLFNVEWCVNARVGTSDPYSVALSNIGDKENE
EYLNFAREKIKNPEGGLYVAVTRLAGITGIVITLCLILIITSSTKTIRRS
YFEVFWYTHHLFVIFFIGLAIHGAERIVRGQTSDSLKEHNLDVCADKIKE
WGKIKECPVPKFAGNPPMTWKWIVGPMFLYLCERLVRFWRSQQKVVITKV
VTHPFKTIELQMKKKGFKMEVGQYIFVKCPQVSKLEWHPFTLTSAPEEDF
FSIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFS
YEVVMLVGAGIGVTPFASILKSVWYKYCDNATSLRLKKIYFYWLCRDTHA
FEWFADLLQLLETQMQERNNANFLSYNIYLTGWDESQANHFAVHHDEEKD
VITGLKQKTLYGRPNWDNEFKTIASQHPNTRIGVFLCGPEALAKTLSKQS
ISNSESGPRGVHFIFNKENF