Norway rat (Rat) ( Rattus norvegicus )
IkBb-C

Western result: +

GST GST-IkBb-C (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: IkBb-C
CBI Antibody Core Code: A0615
"Lot" Number: 1 Position: 100(111-210)
% Identical Homology of Target to Mouse Protein: 92% (NP_035038.2| nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta; I(Kappa)B(beta))
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
EKLYAAGAGVLVTERGGHTALHLACRVRAHTCAYVLLQPR
PSHPRDASDTYLTQSQDHTPDTSHAPVATDPQPNPGNEEE
LRDEDWRLQLEAENYDGHTP

Sequence Name: IkBb-C
Full name: Rat I-kappa-B-beta
UniGene Number: N.A.
Accession Number: NM_030867
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 359
NCBI information page: click here

Full length sequence:
MAGVACLGKTADADEWCDSGLGSLGPDAAAPGGPGLVAELSPELSWAPLV
FGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYLDLQNDLGQTALHL
AAILGEASTVEKLYAAGAGVLVTERGGHTALHLACRVRAHTCAYVLLQPR
PSHPRDASDTYLTQSQDHTPDTSHAPVATDPQPNPGNEEELRDEDWRLQL
EAENYDGHTPLHVAVIHKDAEMVQLLRDAGADLNKPEPTCGRTPLHLAVE
GQAAGVLALLLKAGADPTARMYGGRTPLGSALLRPNPVLARLLRAHGAPE
PEDKDDKLSPCSNSSSDSDSDNRDEGDEYDDIVVHSRRSQNQPPPSPAAK
PLPDDPNPA