Norway rat (Rat) ( Rattus norvegicus )
GRK4

Western result: +/-

GST GST-GRK4 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: GRK4
CBI Antibody Core Code: A0402
"Lot" Number: 1 Position: 100(101-200)
% Identical Homology of Target to Mouse Protein: 85% (NP_062370.1| G protein-coupled receptor kinase 2, groucho gene related; G protein-coupled receptor kinase 4)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
GLSIYSRFFKENSEVSLPQIPPDLVKECKCNLKQSSPSQN
VFQDCAGVIYKYLSEKPFEEYQESTYYNRFLQWKWLERRP
VTKNTFRQYRVLGKGGFGEV

Sequence Name: GRK4
Full name: G protein-coupled receptor kinase
UniGene Number: N.A.
Accession Number: X97568
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 575
NCBI information page: click here

Full length sequence:
MELENFMANTLLLKARQGFTKKTGRSKKWRELLKLPPVSMCSDLRHSIEK
DFSSLCDQQPIGRLLFRQFCNTKPDLKRCIEFLDAAAEYEVTIEEEQREF
GLSIYSRFFKENSEVSLPQIPPDLVKECKCNLKQSSPSQNVFQDCAGVIY
KYLSEKPFEEYQESTYYNRFLQWKWLERRPVTKNTFRQYRVLGKGGFGEV
CACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKRILEKLHSRFVVSLA
YTYETKDALCLVLTIMNGGDLKYHIYNLGNPGFEEQRAVFYAAELCCGLE
DLQRERIVYRDLKPENILLDDHGHIRISDLGLALEIPEGEMVRGRVGTVG
YMAPEIISHEKYTFSPDWWGLGCLIYEMIAGHSPFRKYKEKVNREEMERR
VKTETEEYSERFSENAKSICSMLLTKDPSKRLGCQSDGASAVKQHPIFKD
INFSRLEANMLDPPFCPDPEAIYCKDILDIGQFSVVKGVNLDTNDEIFYT
QFATGCVTIPWQNEMIESGCFKDLNEYEDKGLSPLEKHKICSCILRPKRN
FFHRLFRRAACLNIAHSEEREPTEH