Norway rat (Rat) ( Rattus norvegicus )
Desmuslin

Western result: +

control Desmuslin-pep1 A0554 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: Desmuslin-pep1
CBI Antibody Core Code: A0554
"Lot" Number: 1 Position: 14(1142-1155)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
GPTSPVVEMGVSDT

Western result: ++

GST GST-Desmuslin A0553 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Desmuslin
CBI Antibody Core Code: A0553
"Lot" Number: 1 Position: 100(790-889)
% Identical Homology of Target to Mouse Protein: 85% (NP_997546.1| desmuslin isoform M)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
ETHMAFSMHQRQRTNQPQVNVPHVEEVTEAGDSEGEQSYF
VSTPDEYPGGHDREDDGSVYGQIHIEEESTIRYSWQDEIA
QGTWRRKARGDTGEEKHMKV

Western result: +/-

control Desmuslin-pep2 A0562 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: Desmuslin-pep2
CBI Antibody Core Code: A0562
"Lot" Number: 2 Position: 28(1135-1162)
% Identical Homology of Target to Mouse Protein: 78% (NP_997546.1| desmuslin isoform M)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
TEQVSYGGPTSPVVEMGVSDTRAIRSWT

Sequence Name: Desmuslin
Full name:
UniGene Number: Rn.46362
Accession Number: NM_182737
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 1255
NCBI information page: click here

Full length sequence:
MLSWRLQTSSEKAELQELNARLYEYVCRVRELERENLLLEEELRSRLSRE
DRWAEDQALYAEEARSLRQQLDELSWSTALAEGERDALRRELLDLQREGL
EASTARGRLDAELEAQRRELEEALGARAALEALLGRLEAERRDLDAAHER
QVRELRARASSLTMHFRARATGPAAPPPRLRDVHDSYALLVAESWRESMQ
LYEDEVRELEQALRRGQESRLQAEDEARLCAQEADALRNQALELEQLRAR
LEDELLRMREEYGMQAEERQRVIDSLEDEKDALTLAMADRLREYQELLQV
KTGLSLEVATYRALLEGESNPEILIWTENIENVPRESRNTSYRYTDSVLQ
RKNEKNLFPRRKTPWAAVNHSSTLYSNGPGHLDSQTTTAVGNAARRGFLT
SCYSSSATTSQQKPLEKTVSSQASLRPVIPTHGFLRNTDAQVKTFSHGTK
AEGMGDTLTRRATESVITRESYRGHQGNVAAGAVNSTQSNERTVILGKKL
ETQATKEQERDRSGVVKLKPEEKMFDSKEKASEERNLRWEELTKLDARER
ESQHLRNEAKGKETLTGRSRKEREVPISLEVSRGSKSEVSTIHLQSSGRK
DSANSREREVKTKETKFHLDGKDTAGSLQSDSTTETIAENIVTTILKQFT
QSPGAEENVSSLPDTKVTYLDRKEFPGDRKTKTEIVVESKLTEDVDVSDE
AGLDYLLSKDVKEVGLKGKSTETVIGEMINLGLKGREGRAKVVNVEIVEE
PMSYVGGGKVEFSTPFQVEEVDDVSPSPKGFIEEEEGEGETHMAFSMHQR
QRTNQPQVNVPHVEEVTEAGDSEGEQSYFVSTPDEYPGGHDREDDGSVYG
QIHIEEESTIRYSWQDEIAQGTWRRKARGDTGEEKHMKVLEVPSASLGGA
FGSAHVKEEASGELHAEPTIIEKEIKIPHEFHTSIKGVFSSEPRHQLVEV
IGQLEETLPERMKEELSALTRQSQGEPGSVSVDVKKVQSSAGGSVTLMAE
VNLSQTVDADQLDLEELSRDEAGEIEKAVESVVRESLTKRSSPVPRSPDR
EDGEEAPAGGFLFKRWATRELYSPSGEKDDTDRVFHSSDQRVTQGPVSAT
VEVTSPTGFVQSHVLEDVSQSVRHVKLGPTEMWRTEQVSYGGPTSPVVEM
GVSDTRAIRSWTRDTGSEVEAHSVSHHGGWRVAHSRDEQSASTSSQASAG
DAHQARGEKGTEQAGFDKTVQLQRMVDQRSVASDEKKVALLYLDNEEEEE
GEGWF