Norway rat (Rat) ( Rattus norvegicus )
rAdra2b (RATA2bR)

Western result: ++++

GST GST-rAdra2b (RATA2bR) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: rAdra2b (RATA2bR)
CBI Antibody Core Code: A0339
"Lot" Number: 1 Position: 100(201-300)
% Identical Homology of Target to Mouse Protein: 77% (NP_033763.2| adrenergic receptor, alpha 2b; subtype alpha2-C2)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
YVIAKRSHCRGLGAKRGSGEGESKKPQPVAGGVPTSAKVP
TLVSPLSSVGEANGHPKPPREKEEGETPEDPEARALPPTW
SALPRSGQGQKKGTSGATAE

Sequence Name: rAdra2b (RATA2bR)
Full name: Alpha-2B-adrenergic receptor (RNG-alpha-2)
UniGene Number: N.A.
Accession Number: M32061.1
Source organism: Norway rat (Rat) ( Rattus norvegicus )
Full length size (aa): 453
NCBI information page: click here

Full length sequence:
MSGPTMDHQEPYSVQATAAIASAITFLILFTIFGNALVILAVLTSRSLRA
PQNLFLVSLAAADILVATLIIPFSLANELLGYWYFWRAWCEVYLALDVLF
CTSSIVHLCAISLDRYWAVSRALEYNSKRTPCRIKCIILTVWLIAAVISL
PPLIYKGDQRPDARGLPQCELNQEAWYILASSIGSFFAPCLIMILVYLRI
YVIAKRSHCRGLGAKRGSGEGESKKPQPVAGGVPTSAKVPTLVSPLSSVG
EANGHPKPPREKEEGETPEDPEARALPPTWSALPRSGQGQKKGTSGATAE
EGDEEDEEEVEECEPQTLPASPASVCNPPLQQPQTSRVLATLRGQVLLGK
NVGVASGQWWRRRTQLSREKRFTFVLAVVIGVFVVCWFPFFFSYSLGAIC
PQHCKVPHGLFQFFFWIGYCNSSLNPVIYTVFNQDFRRAFRRILCRPWTQ
TGW