malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
pfG377

Western result: +/-

GST GST-pfG377 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: pfG377
CBI Antibody Core Code: A0471
"Lot" Number: 1 Position: 100(590-689)
% Identical Homology of Target to Mouse Protein: 33% (NP_852658.1| importin 7; RAN binding protein 7)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
GRVLSDDHDIDSKEYTENYFTPEEEEQALKDFKEDEASVH
HLDHYDNNMDPYYEYQGEFGSYEEEEDELRKFKEDEPEPW
PLDESPHIEHGTLHDDELNP

Sequence Name: pfG377
Full name:
UniGene Number: N.A.
Accession Number: PFL2405c
Source organism: malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
Full length size (aa): 3119
NCBI information page: click here

Full length sequence:
MKLLLFFFISSIFLQLTSGKRILNFDNIIKHLKESKLLPEDIPHVLENDI
IIVPPYLIYKYKGKIYHLHNNVDLTLINHPEEDSCDKEEIWESPFPPKVP
EPERPHEPEMEPQVEPEPGPLPEEVREPEPEPEAEPEKELEMEEQEEVIE
ADMVLDEETGIKIPKKTEQDVLEVSKFREEYELPNVVELTPEEKEKNNIL
HFAGNKSTAFNLKEIINYKKDESLMNSLSSSFDHFYTPNVEERNLSNAYN
VDINDYYDLLRALHILCKDEDNKLYTINRIPKDELLFFLKHAYVNIYNSL
KKYILLNGYNFEDYIYTSENFTLDQIFKDYFFLSNDDTNENGSFNNIIES
IKYIKKAIEKLNVKRIEEKIKYFFQIYEVHAFDFKLLHYIFSRNQLLNYS
EDDLIHHAVDIMNITRIDISPRVISALFMYFLNKVNIFLVPQYVTRNRND
FTLLLEHNDLLLRTEIIYRDFLKHFFKHKTPHVHLKKNNHDNAYQLVPWS
QVLFSEFNSTYDFLNFKYMIILFHDSYHAFVDYFEGDDKLNQVLNDNKEN
KTIDNLDKFFNELLNLFLIENVGILSTLEEYFVSTVKQVGRVLSDDHDID
SKEYTENYFTPEEEEQALKDFKEDEASVHHLDHYDNNMDPYYEYQGEFGS
YEEEEDELRKFKEDEPEPWPLDESPHIEHGTLHDDELNPFQEEKKDDESF
YTSEDEDTLDHSHEKEDDVSHPEHEDHNEGYIPHTYDDDSFFTDEEDDEG
DEVNEENVPLNHEEENVPLNHEEDNFPLNHEEDNFPLNHEEENVPLNHEE
ENVPLNHEEENVPLNHEEENYNSFLSYQKYDERDISPHNVNQHKKEIFHH
RNITPYHVNHHNQHMDQHDHHIDHHNHHIDHHNHHIDHHNHHIDHHNHHI
DHQDHHIDHHNHHIDHHNHHIDHHDHHIDHHDHHIDHHNHHIDHKSNNQF
LQKHQNYVRGHSSFITISEGEENHDNRELRKKIEANLKEEWKKRFNEQQE
QRERKKKAEEDEMNETIQKHDMETSKLEKKEEVDEVTQDEEFDKQYAIDD
QEELEFLRTRDSEGSESDVPKDKVKPFPDGRSPDSFYYNTAISSFHEKME
ELYNTSISSSLNYVKEINRKFDDVYKELKSKTYPKFDDLTSQTKTNCNKL
FQKLNETIKDKEYQKNIQSYKNKVIDILDDIQKKANGKYIIIQNLIIEKI
DIYKGDVVRLSDRKFYKNFRKVLGKRKMKMLEDFRAQFKGAIRFIKDLTT
TYMEEEYTKVLEDIYMEKKKYYKEEYSKMRRIISSNLDYEVNKQIKEHYH
KVDTISEHKFQEIRQHMRDKIENTIHELYKEMYVQIQIDLTNYYHQLENI
HSELLQALQQNKNIPRHLNVLEKKLEITKRKKKNKPDISTSSHATDEQQV
SDTLIRGAHNHGDIIKGEDNDEVLLIEQIQSLKTKMGDNQNQVGSILEKL
NNLSDQYQLLQDKLNVVEDIYKNLRNFKHYIEKLHKESKINREKFITKVD
VLSNVYSTLEYMVKFLLHDFQEWSFEKDELEKHLYELEERKKYITLEIQI
RDTLSTNIQNGEGDHINNNNNNNNVRNNLKKQVLKDLDLEISKLKGHILE
VDIKKNIALEQINYLTNNTNETVPDVIRDLMPAPRIVPVSEDIYDYITWV
RDNTAVINNTLRHFVMTFDQKIYDYDDHLIFVYNIKELIYKENLADEKYN
ANYYYEMNRFYLHLEEFFYILKHYVELRKIQDLGEVAIPSFEENIKTKGS
LIDVHQKDKDGTQIIEYLERNVLHIYSILRNIKNNNIHLYHYLVPDLSNI
LIDPEEFKKMSNGEQPELNKLHKYLLDAYTNLKKEGQVVDQIYVEYINFH
NHFEHTYDKLLWYKTILKRYIERSKKIYEKSYGTYTVSHIQLYDNKKDGH
DNHHDQHHDNHHDQHHDHHHDQHHDHHHDHDQHHNNNYNYNNISFLRSFV
QLNYHNTLHERSDHIFLKSPILLQYDPEKIVESNEKRHIRVIRDQNNFPY
KLGEVKETYVLNKSIHDKFVKSKKLDEYKKYMNNKLYDIVHEHKRGKGKL
YLFEKEELEKYQLIFGGKTLIKGSLLNFLSHININLNKNLLINQINDTIN
EYLKKRNLSYNSIIELIKYLQKNPSVIHFIDLIKLGKNILERSNVLYIKK
EWFTLYLYTLLELLGIEVNMEHVDVHEERGLKNEHNNYISEKSYALHYLY
VLRNYNKRIEIKNQIQNYVLQFLLPEHKFDMEDYQPILYEFLKAINDSMD
GDSITYNEFKKKNPEVYTTLVSVIKKGIEDFFYTMFNKIIEEHPELKEEI
YFDDNYNDEEKDRFRYANYTWERANLNEILTLYFYVLNPGLRNVIYKHFI
KVNIKCLKDYKNFDFKEIFSFLNYDDIGATFLNYLKEHELFEIPPLIIFL
RFFFHEKNEELFTFNSIRYKINSYFEGKSLDRKTKEMIARLVYSFFMRIS
YLSVSYFDHDIFKIINKSDLNIIILFHTIINKINYLLYCGTEKLFIERYA
PTNLFVQNRIFKIDAVKVFHHFIYSLPPYMRNVVFHFFKSNQFIDVTKKL
IIKFLQHSHSLLDITNHKDGHFYQLKNNTYKLETFLFSWKYNFTKNKNII
EPTFTKFIENKPVFKNMPLLYNRRIKYDSINEKLQYKEELDLHDMIDNES
FHQLLDLLNKESITLEQLSFLFSRYITEHANIKDFPKLFMFKKYVTYVQD
EKFFINLYEYLYNYLHKEFGLPQPIYELYYVFIKTFIEYILLPEKLTQEE
KSQNVLNYDLQNKYMNFHHFVQKLCSCLFNFYYYDEIYECQPFGFFTKEE
FSYEQQIFNILQQPFINISPIIKHNYIHDDYYMYEKDLNGNYLSSNINNN
NNNNNINNIDSNYVNRRDKNGDYIHGTNINSDILYHFIKQYIPLVEPSDE
EHLQIEVFKFKYFTLEITKFVLRHILKKLPFNYLIHMNWNDTYIHIDHII
NNLFLHFFKKNKAHLLQQNQLQGLTHKINFENNLGKDHTKDEYLSKHIPQ
SAQNYDRNKFSGTINNLLKDPNNLPPTYRHIKYYMDHYILQYSLHNHMLF
KYLHNIFQHIDQYKTYFPTLAELYLYIDKRVTIINVINNSFLYKRIHTRD
VIHTFLLLCHEIGLDLYEL