malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
1TM

Western result: +

GST GST-1TM (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: 1TM
CBI Antibody Core Code: A0492
"Lot" Number: 1 Position: 100(38-137)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
HIIDNTKGKKNDNDKKYEQLNNVFSLNKQKILSNLNYIKE
KKTLSYLLLSYNNKKKKAILKRKIKRQECYEKKYEETNKE
NEHRYINIYKMENKYKNHIN

Sequence Name: 1TM
Full name:
UniGene Number: N.A.
Accession Number: PFD0820w
Source organism: malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
Full length size (aa): 275
NCBI information page: click here

Full length sequence:
MNIVLQIFLVLSFYQIICFFLAHYNPYFTTNIVKCDIHIIDNTKGKKNDN
DKKYEQLNNVFSLNKQKILSNLNYIKEKKTLSYLLLSYNNKKKKAILKRK
IKRQECYEKKYEETNKENEHRYINIYKMENKYKNHINKNFIFLEISKEEL
NMLDENLELHGSNLNLPLKNPKLSQQYDQVLFLEKNVTWYEMPTPPISCR
QTGCPHTYQICAPMYVDNNKFENTTILKIIQEALNISSRKEIKFPNIDIH
NSYELVYTCICKKYAQNGFCDETIV