malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
RH4

Western result: +

GST GST-RH4 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: RH4
CBI Antibody Core Code: A1110a
"Lot" Number: 1 Position: 100 (1111-1210)
% Identical Homology of Target to Mouse Protein: 30% (XP_485385.1| hypothetical protein XP_485385)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
RTSHTHDNNRIETTAENNIGGLSNSNVHDGRDSQRNRMHI
NSRSRHGSLESDIVVRGDDISNIEGGEEEEEDANTLKYPR
NVLNNKNSRTYNIEEYIYRD

Sequence Name: RH4
Full name: PFD1155w
UniGene Number: N.A.
Accession Number: AF420309
Source organism: malaria parasite (malaria (P. falciparum)) ( Plasmodium falciparum )
Full length size (aa): 1388
NCBI information page:

Full length sequence:
MRKTLKLFYISGFLFSSRALCKLENYIKNEYDRENSFYILNNKVEKEYEK
GTNNNINIDNKKEALLEDKKNVDNKDIKEQLPENNKNFEEKILHEDICKL
NKENIEKEGLYKPNTFKSIGNDLLYKKGKLNFLYEYGMELLHINKVPMNR
MNNSRGLGDSNMSFLDISNNNMNSLNSMNNLNNSNNFDSSNSLSFVDMKS
IHRCIKKRAKGERDWACNDKNTKEPNICVSDRRVQLCTGNLIELPINDST
KEKFKEKLILAAQKEGSLLFEKFGKKYNEEFCLNLKWSYGDYGDIIKGTD
MEGLGRSIKVEFEIDRIFKRDNKKSAENRKKWWEEISTHIWNAMIKEHKE
KFPKDVKECSADKPLDEPQINRWLMEWAYDTDYNKNLWYNKLDDSKNKCN
RICPKKRNFSLYKTAFEYLKQKWDKYKELNFSSIFDQLNAKYYNKCICQN
NKIENNALYVKIEDICNNTKVKSIYGELYCKEKGNDKIWQCINEHIKDFP
DVCGPPRRQQLCLGNLDKDEFKNVNDLKKFLNEIILGIRDEGKFLIEKYR
KNMHENMYLDERACKYLNYSFDDYKNIILGKDMWRDPNSIKTENILKGNF
EGIKANIVSMYPSYADLSLDEFRKHWWDQNKKQLWEAISCEFYKGNHTGV
CLMEDDNDNQYLHWFREWKNDFCIDKLKWNDVIKEPCIDKKVKSPKPSEN
PSDVATVCNKSCTDYDKWIINKRKEYKMQSSKYKRDRSLFNNVIQNLKPW
EYLSMKCTECTCNLDTQTFVYPYKGYEDICKSTVKPYDPEDIKDEEFNEP
SLNVNPLSLTSQDVTERVSSVDDVLSIKENVDLKPFKPKGGTQSSHVDQV
GNPRESESKPSGANGREDPSTESSTYNDGVITSSSSLGSSSGRDVSSSPV
GVGDEHEAKELLPPQKIIDGVTQSDESTLSQHGKESSQEQHNLDGSSLSR
HSNQDEERSIITSDVEHGTNSLFGSQIQDQETILGESEPLTTSPPEHETS
KMDTHAGGKNMEQVRNASVDSSSEMSNGGRGGLKTKEMKGEEVTGITSKN
DINLEDSTVHSRQNKIENSGDNTQGKEHINVLQGMDKHLENPPTSERGDS
VLESEFSKLNRTSHTHDNNRIETTAENNIGGLSNSNVHDGRDSQRNRMHI
NSRSRHGSLESDIVVRGDDISNIEGGEEEEEDANTLKYPRNVLNNKNSRT
YNIEEYIYRDVNKVADDIMRSYKSNRCTNNLSSNYCSKLKKESLSNTCTN
EDSKRLCCSISDYCMKFFNFNSSGYHSCMRKEFSNHAYKCFAGKGFSNMY
YFAGGGLFFIILFLLGAKSSILNWSEDNELNGTAFEEANFEDAAFEVTTG
QDSAFEDYDENAYRIGLLMNQKIQSAKSSDYLEYHVDN