deer mouse ( Peromyscus maniculatus )
CD4

Antibody Name/Abbreviation: CD4
CBI Antibody Core Code: A0889
"Lot" Number: 2 Position: 100(183-282)
% Identical Homology of Target to Mouse Protein: 63% (NP_038516.1| CD4 antigen)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
TLTLAKGTLHQDVNLVVMKLAQKDNAVTCEVRGPTSPKMK
LTLKLENQDKVSGQEKVVEMKDPEAGQWLCELNEGDELKI
SSKIQVSSRGLKQDQPTFLA

Other western:
Code: A0889
Lot: 1

Western result: -

GST GST-CD4 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: CD4
Full name: CD4
UniGene Number: N.A.
Accession Number: N.A.
Source organism: deer mouse ( Peromyscus maniculatus )
Full length size (aa): 314
NCBI information page:

Full length sequence:
LKEEXELWVFRVTASPDTRLLQGQSLTLTLDSSKVTHPSIECKGPGNSIV
KGSKTLSMPNLRIQDSGIWTCTVTQSQHKNNFDINISVLGFQKTSITVYK
NEGELAEFSFPLNFGEENLRGELRWRAEKAPSPQPWITFSLENKKVSMQK
TKDNLKPQMEESLPLRLKIPQVSLESAGSGNLTLTLAKGTLHQDVNLVVM
KLAQKDNAVTCEVRGPTSPKMKLTLKLENQDKVSGQEKVVEMKDPEAGQW
LCELNEGDELKISSKIQVSSRGLKQDQPTFLALVLGGIFSFLTFIGLCIL
CCVRCRHQQRQAER