deer mouse ( Peromyscus maniculatus )
CD3g

Western result: +

control CD3g (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: CD3g
CBI Antibody Core Code: A0872
"Lot" Number: 1 Position: 20(162-181)
% Identical Homology of Target to Mouse Protein: 85% (NP_033980.1| CD3 antigen, gamma polypeptide)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
LKDRDDDQYSHLQGNQRRKK

Sequence Name: CD3g
Full name: CD3 gamma
UniGene Number: N.A.
Accession Number: N.A.
Source organism: deer mouse ( Peromyscus maniculatus )
Full length size (aa): 181
NCBI information page:

Full length sequence:
MVKGLTGLVLVIFLLQGTVVQPKQENQNHLVKVDDNRGDDSVFLTCGLKD
KTITWLKDGHAISSNVTKHTWNLGSSAKDPRGMYWCQGPTGKSKPLQVYY
RMCENCIELNIGTISGFIFAEIISIFFLAVGVYFIAGQDGVRQSRASDKQ
TLLPNEQLYQPLKDRDDDQYSHLQGNQRRKK