Ashe juniper ( Juniperus ashei )
Jun-a2

Western result: +-

GST GST-Jun-a2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Jun-a2
CBI Antibody Core Code: A0384
"Lot" Number: 1 Position: 100(101-200)
% Identical Homology of Target to Mouse Protein: 40% (NP_955011.1| endothelin converting enzyme 1)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
KFVVNNLVFYGPCQPHFSFKVDGTIAAYPDPAKWKNSKIW
MHFARLTDFNLMGTGVIDGQGNRWWSDQCKTINGRTVCND
KGRPTAIKIDFSKSVTVKEL

Sequence Name: Jun-a2
Full name: pollen major allergen 2
UniGene Number: N.A.
Accession Number: AJ404653
Source organism: Ashe juniper ( Juniperus ashei )
Full length size (aa): 507
NCBI information page: click here

Full length sequence:
MSMKFMAALAFLALQLIVMAAGEDQSAQIMLDSDTKQYHRSSRNLRKRVH
HARHDVAIVFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANK
KFVVNNLVFYGPCQPHFSFKVDGTIAAYPDPAKWKNSKIWMHFARLTDFN
LMGTGVIDGQGNRWWSDQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKEL
TLTNSPEFHLVFGECDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKC
TIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSRSEVSFVHL
DGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTS
AAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSNIKLSNVF
LKLTSGKVATCVNKNANGYYTNPLNPSCKSLHPGRTPKELELHQKPTTLL
MDEKMGASLNSSPPNCKNKCKGCQPCKPKLIIVHPNQPEDYYPQRWVCSC
HNKIYNP