Ashe juniper ( Juniperus ashei )
Jun-a1

Western result: +/-

GST GST-Jun-a1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Jun-a1
CBI Antibody Core Code: A0383
"Lot" Number: 2 Position: 100(237-336)
% Identical Homology of Target to Mouse Protein: 33% (NP_032351.1| iduronidase, alpha-L-; alpha-L-iduronidase)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
TVAFNQFGPNAGQRMPRARYGLVHVANNNYDPWNIYAIGG
SSNPTILSEGNSFTAPSESYKKEVTKRIGCESPSACANWV
WRSTRDAFINGAYFVSSGKT

Sequence Name: Jun-a1
Full name: pollen major allergen 1-1
UniGene Number: N.A.
Accession Number: AF106662
Source organism: Ashe juniper ( Juniperus ashei )
Full length size (aa): 367
NCBI information page: click here

Full length sequence:
MASPCLIAVLVFLCAIVSCYSDNPIDSCWRGDSNWDQNRMKLADCAVGFG
SSTMGGKGGDFYTVTSTDDNPVNPTPGTLRYGATREKALWIIFSQNMNIK
LKMPLYVAGHKTIDGRGADVHLGNGGPCLFMRKVSHVILHSLHIHGCNTS
VLGDVLVSESIGVEPVHAQDGDAITMRNVTNAWIDHNSLSDCSDGLIDVT
LGSTGITISNNHFFNHHKVMLLGHDDTYDDDKSMKVTVAFNQFGPNAGQR
MPRARYGLVHVANNNYDPWNIYAIGGSSNPTILSEGNSFTAPSESYKKEV
TKRIGCESPSACANWVWRSTRDAFINGAYFVSSGKTEETNIYNSNEAFKV
ENGNAAPQLTKNAGVVT