human ( Homo sapiens )
2-HPCL

Western result: +

GST GST-2-HPCL A0956 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: 2-HPCL
CBI Antibody Core Code: A0956
"Lot" Number: 1 Position: 100 (327-426)
% Identical Homology of Target to Mouse Protein: 87% (NP_064359.2| 2-hydroxyphytanoyl-CoA lyase)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
VTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKEL
ASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRT
VLQNYLPRHRLDAGTFGTMG

Antibody Name/Abbreviation: 2-HPCL-pep-test
CBI Antibody Core Code: A0957
"Lot" Number: 1 Position: 20 (333-352)
% Identical Homology of Target to Mouse Protein: 75% (NP_064359.2| 2-hydroxyphytanoyl-CoA lyase)
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
EELDKTPWQYPPESKWWKTL

Sequence Name: 2-HPCL
Full name: 2-hydroxy-phytanoyl-CoA lyase
UniGene Number: N.A.
Accession Number: N.A.
Source organism: human ( Homo sapiens )
Full length size (aa): 578
NCBI information page:

Full length sequence:
MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQL
GIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANANM
NCWPLLVIGGSSERNQETMGAFQEFPQVEACRLYTKFSARXSSIEAIPFV
IEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAET
SAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMG
KGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVK
FIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWW
KTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGAN
TMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGHWII
CVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKF
QDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLEQSLADTT
KPSLINIMIEPQATRKAQDFHWLTRSNM