human ( Homo sapiens )
Pak2

Western result: +

GST GST-Pak2 (fragment)

32.5 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Pak2
CBI Antibody Core Code: A0927
"Lot" Number: 1 Position: 50(fused:47-65|143-156|205-221)
% Identical Homology of Target to Mouse Protein: 94% (NP_796300.1| p21-activated kinase 2)
Molecular Weight of fusion protein: 32.5 KDa

Target sequence used to make this antibody:
KKPRHKIISIFSGTEKGSKTPPEKDGLPSGTPAPVGDSHV
DGAAKSLDKQ

Sequence Name: Pak2
Full name: p21-activated protein kinase 2
UniGene Number: N.A.
Accession Number: U24153
Source organism: human ( Homo sapiens )
Full length size (aa): 525
NCBI information page:

Full length sequence:
MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPR
HKIISIFSGTEKGSKKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPE
QWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQKYLSFTPPEKDGL
PSGTPALNAKGTEAPAVVTEEEDDDEETAPPVIAPRPDHTKSIYTRSVID
PVPAPVGDSHVDGAAKSLDKQKKKPKMTDEEIMEKLRTIVSIGDPKKKYT
RYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKE
LKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETACMDEAQIAAVC
RECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSK
RSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPL
RALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPF
LKLAKPLSSLTPLIMAAKEAMKSNR