human ( Homo sapiens )
TERT

Western result: +

GST GST-TERT (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TERT
CBI Antibody Core Code: A0913
"Lot" Number: 1 Position: 100(244-343)
% Identical Homology of Target to Mouse Protein: 30% (XP_152907.3| RIKEN cDNA 5430405G24)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
PEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEAT
SLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPP
VYAETKHFLYSSGDKEQLRP

Sequence Name: TERT
Full name: telomerase
UniGene Number: N.A.
Accession Number: NP_003210
Source organism: human ( Homo sapiens )
Full length size (aa): 1132
NCBI information page:

Full length sequence:
mpraprcravrsllrshyrevlplatfvrrlgpqgwrlvqrgdpaafral
vaqclvcvpwdarpppaapsfrqvsclkelvarvlqrlcergaknvlafg
falldgarggppeafttsvrsylpntvtdalrgsgawglllrrvgddvlv
hllarcalfvlvapscayqvcgpplyqlgaatqarppphasgprrrlgce
rawnhsvreagvplglpapgarrrggsasrslplpkrprrgaapepertp
vgqgswahpgrtrgpsdrgfcvvsparpaeeatslegalsgtrhshpsvg
rqhhagppstsrpprpwdtpcppvyaetkhflyssgdkeqlrpsfllssl
rpsltgarrlvetiflgsrpwmpgtprrlprlpqrywqmrplflellgnh
aqcpygvllkthcplraavtpaagvcarekpqgsvaapeeedtdprrlvq
llrqhsspwqvygfvraclrrlvppglwgsrhnerrflrntkkfislgkh
aklslqeltwkmsvrdcawlrrspgvgcvpaaehrlreeilakflhwlms
vyvvellrsffyvtettfqknrlffyrksvwsklqsigirqhlkrvqlre
lseaevrqhrearpalltsrlrfipkpdglrpivnmdyvvgartfrrekr
aerltsrvkalfsvlnyerarrpgllgasvlglddihrawrtfvlrvraq
dpppelyfvkvdvtgaydtipqdrlteviasiikpqntycvrryavvqka
ahghvrkafkshvstltdlqpymrqfvahlqetsplrdavvieqssslne
assglfdvflrfmchhavrirgksyvqcqgipqgsilstllcslcygdme
nklfagirrdglllrlvddfllvtphlthaktflrtlvrgvpeygcvvnl
rktvvnfpvedealggtafvqmpahglfpwcgllldtrtlevqsdyssya
rtsirasltfnrgfkagrnmrrklfgvlrlkchslfldlqvnslqtvctn
iykilllqayrfhacvlqlpfhqqvwknptfflrvisdtaslcysilkak
nagmslgakgaagplpseavqwlchqafllkltrhrvtyvpllgslrtaq
tqlsrklpgttltaleaaanpalpsdfktild