human ( Homo sapiens )
LGR8/GREAT

Antibody Name/Abbreviation: LGR8/GREAT
CBI Antibody Core Code: A0893
"Lot" Number: 1 Position: 100(83-182)
% Identical Homology of Target to Mouse Protein: 75% (NP_536716.1| leucine-rich repeat-containing G protein-coupled receptor 8; G protein coupled receptor affecting testicular descent; G protein-coupled receptor 106)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
TSGWATIFGTVHGNANSVALTQECFLKQYPQCCDCKETEL
ECVNGDLKSVPMISNNVTLLSLKKNKIHSLPDKVFIKYTK
LKKIFLQHNCIRHISRKAFF

Antibody Name/Abbreviation: LGR8/GREAT-pep2
CBI Antibody Core Code: A0894
"Lot" Number: 1 Position: 100(37-136)
% Identical Homology of Target to Mouse Protein: 79% (NP_536716.1| leucine-rich repeat-containing G protein-coupled receptor 8; G protein coupled receptor affecting testicular descent; G protein-coupled receptor 106)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
QGSMITPSCQKGYFPCGNLTKCLPRAFHCDGKDDCGNGAD
EENCGDTSGWATIFGTVHGNANSVALTQECFLKQYPQCCD
CKETELECVNGDLKSVPMIS

Antibody Name/Abbreviation: LGR8/GREAt-pep3
CBI Antibody Core Code: A0895
"Lot" Number: 2 Position: 100(281-380)
% Identical Homology of Target to Mouse Protein: 78% (NP_536716.1| leucine-rich repeat-containing G protein-coupled receptor 8; G protein coupled receptor affecting testicular descent; G protein-coupled receptor 106)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
DSLTVLFLPRNQIGFVPEKTFSSLKNLGELDLSSNTITEL
SPHLFKDLKLLQKLNLSSNPLMYLHKNQFESLKQLQSLDL
ERIEIPNINTRMFQPMKNLS

Other western:
Code: A0895
Lot: 1

Western result: ++//- ?

GST GST-LGR8/GREAt-pep3 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: LGR8/GREAT
Full name:
UniGene Number: N.A.
Accession Number: NP_570718
Source organism: human ( Homo sapiens )
Full length size (aa): 754
NCBI information page:

Full length sequence:
MIVFLVFKHLFSLRLITMFFLLHFIVLINVKDFALTQGSMITPSCQKGYF
PCGNLTKCLPRAFHCDGKDDCGNGADEENCGDTSGWATIFGTVHGNANSV
ALTQECFLKQYPQCCDCKETELECVNGDLKSVPMISNNVTLLSLKKNKIH
SLPDKVFIKYTKLKKIFLQHNCIRHISRKAFFGLCNLQILYLNHNCITTL
RPGIFKDLHQLTWLILDDNPITRISQRLFTGLNSLFFLSMVNNYLEALPK
QMCAQMPQLNWVDLEGNRIKYLTNSTFLSCDSLTVLFLPRNQIGFVPEKT
FSSLKNLGELDLSSNTITELSPHLFKDLKLLQKLNLSSNPLMYLHKNQFE
SLKQLQSLDLERIEIPNINTRMFQPMKNLSHIYFKNFRYCSYAPHVRICM
PLTDGISSFEDLLANNILRIFVWVIAFITCFGNLFVIGMRSFIKAENTTH
AMSIKILCCADCLMGVYLFFVGIFDIKYRGQYQKYALLWMESVQCRLMGF
LAMLSTEVSVLLLTYLTLEKFLVIVFPFSNIRPGKRQTSVILICIWMAGF
LIAVIPFWNKDYFGNFYGKNGVCFPLYYDQTEDIGSKGYSLGIFLGVNLL
AFLIIVFSYITMFCSIQKTALQTTEVRNCFGREVAVANRFFFIVFSDAIC
WIPVFVVKILSLFRVEIPDTMTSWIVIFFLPVNSALNPILYTLTTNFFKD
KLKQLLHKHQRKSIFKIKKKSLSTSIVWIEDSSSLKLGVLNKITLGDSIM
KPVS