human ( Homo sapiens )
TIF1beta

Western result: +

GST GST-TIF1beta (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TIF1beta
CBI Antibody Core Code: A0879
"Lot" Number: 1 Position: 100(420-519)
% Identical Homology of Target to Mouse Protein: 95% (NP_035718.1| tripartite motif protein 28; transcriptional intermediary factor 1, beta)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
PAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYS
SAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTA
DSQPPVFKVFPGSTTEDYNL

Sequence Name: TIF1beta
Full name:
UniGene Number: N.A.
Accession Number: X97548
Source organism: human ( Homo sapiens )
Full length size (aa): 835
NCBI information page:

Full length sequence:
MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASS
PAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAA
NSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDAN
QCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKS
RDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVR
NQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAIL
QIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFAS
WALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTK
SAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYG
FGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTAD
SQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGM
AIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTS
SGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLD
CHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPAN
QRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEK
LSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAF
GDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP