human ( Homo sapiens )
GGT1

Western result: +

control GGT1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: GGT1
CBI Antibody Core Code: A0869
"Lot" Number: 1 Position: 20(46-65)
% Identical Homology of Target to Mouse Protein: 70% (NP_032142.1| gamma-glutamyltransferase 1; gamma-glutamyl transpeptidase)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
DAKQCSKIGRDALRDGGSAV

Sequence Name: GGT1
Full name: Gamma-glutamyltranspeptidase 1 precursor (EC 2.3.2.2) (Gamma-glutamyltransferase 1) (CD224 antigen)
UniGene Number: Hs.352119
Accession Number: NM_013430
Source organism: human ( Homo sapiens )
Full length size (aa): 569
NCBI information page:

Full length sequence:
MKKKLVVLGLLAVVLVLVIVGLCLWLPSASKEPDNHVYTRAAVAADAKQC
SKIGRDALRDGGSAVDAAIAALLCVGLMNAHSMGIGGGLFLTIYNSTTRK
AEVINAREVAPRLAFATMFNSSEQSQKGGLSVAVPGEIRGYELAHQRHGR
LPWARLFQPSIQLARQGFPVGKGLAAALENKRTVIEQQPVLCEVFCRDRK
VLREGERLTLPQLADTYETLAIEGAQAFYNGSLTAQIVKDIQAAGGIVTA
EDLNNYRAELIEHPLNISLGDAVLYMPSAPLSGPVLALILNILKGYNFSR
ESVESPEQKGLTYHRIVEAFRFAYAKRTLLGDPKFVDVTEVVRNMTSEFF
AAQLRAQISDDTTHPISYYKPEFYTPDDGGTAHLSVVAEDGSAVSATSTI
NLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQPL
SSMCPTIMVGQDGQVRMVVGAAGGTQITTATALAIIYNLWFGYDVKRAVE
EPRLHNQLLPNVTTVERNIDQAVTAALETRHHHTQIASTFIAVVQAIVRT
AGGWAAASDSRKGGEPAGY