human ( Homo sapiens )
P2X5b

Western result: +

GST GST-P2X5b A0861 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: P2X5b
CBI Antibody Core Code: A0861
"Lot" Number: 1 Position: 100(285-384)
% Identical Homology of Target to Mouse Protein: 55% (NP_201578.1| purinergic receptor P2X5)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
RTLMKAYGIRFDVMVNGKGASFCDLVLIYLIKKREFYRDK
KYQEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQ
QELQEPPEANVGSSSQKGNG

Western result: +

control P2X5b-pep4 A0866 (fragment)

29.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: P2X5b-pep4
CBI Antibody Core Code: A0866
"Lot" Number: 1 Position: 20(315-334)
% Identical Homology of Target to Mouse Protein: 60% (NP_201578.1| purinergic receptor P2X5)
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
IKKREFYRDKKYQEVRGLED

Antibody Name/Abbreviation: P2X5b-pep5
CBI Antibody Core Code: A0867
"Lot" Number: 1 Position: 20(365-384)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
QELQEPPEANVGSSSQKGNG

Antibody Name/Abbreviation: P2X5b-fuse5x20
CBI Antibody Core Code: A0862
"Lot" Number: 1 Position: 100 fuse (94-113;120-139;252-271;315-334;365-384)
% Identical Homology of Target to Mouse Protein: 64% (NP_201578.1| purinergic receptor P2X5)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
PAENEGIPDGACSKDSDCHAGNGVKTGRCLRRENLARGTC
SFSRLDNKLSKSVSSGYNFRIKKREFYRDKKYQEVRGLED
QELQEPPEANVGSSSQKGNG

Sequence Name: P2X5b
Full name: P2X purinoceptor 5 (ATP receptor) (P2X5) (Purinergic receptor)
UniGene Number: N.A.
Accession Number: HSU49396; U49396
Source organism: human ( Homo sapiens )
Full length size (aa): 397
NCBI information page:

Sequence Notes: A0866: This antibody was generated from mice that were immunized with a mixed peptides from different regions of the antigen sequence. Serum was tested on individual peptide.

Full length sequence:
MGQAGCKGLCLSLFDYKTEKYVIAKNKKVGLLYRLLQASILAYLVVWVFL
IKKGYQDVDTSLQSAVITKVKGVAFTNTSDLGQRIWDVADYVIPAENEGI
PDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETS
SRPEEPFLKEAEDFTIFIKNHIRFPKFNFSNNVMDVKDRSFLKSCHFGPK
NHYCPIFRLGSVIRWAGSDFQDIALEGGVIGINIEWNCDLDKAASECHPH
YSFSRLDNKLSKSVSSGYNFRFARYYRDAAGVEFRTLMKAYGIRFDVMVN
GKGASFCDLVLIYLIKKREFYRDKKYQEVRGLEDSSQEAEDEASGLGLSE
QLTSGPGLLGMPEQQELQEPPEANVGSSSQKGNGSVCPQLLEPHRST