human ( Homo sapiens )
DRIP150 (CRISP150, TRAP170)

Antibody Name/Abbreviation: DRIP150 (CRISP150, TRAP170)
CBI Antibody Core Code: A0572
"Lot" Number: 2 Position: 100(1071-1170)
% Identical Homology of Target to Mouse Protein: 32% (NP_076161.2| single-stranded DNA-binding protein isoform a; Lck-associated signal transducer)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWP
GSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTS
SQTMPTNMPPPRKLPQRSWA

Other western:
Code: A0572
Lot: 1

Western result: ++

GST GST-DRIP150 (CRISP150, TRAP170) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: DRIP150 (CRISP150, TRAP170)
Full name: vitamin D receptor-interacting protein complex component DRIP150; cofactor required for Sp1 transcriptional activation, subunit 2,
UniGene Number: N.A.
Accession Number: NP_004220
Source organism: human ( Homo sapiens )
Full length size (aa): 1454
NCBI information page: click here

Full length sequence:
MAPVQLENHQLVPPGGGGGGSGGPPSAPAPPPPGAAVAAAAAAAASPGYR
LSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLL
ALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLP
SFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILR
HRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKL
EILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFC
LSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTG
TASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKL
LIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSE
CLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWL
GQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYI
VVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVF
RTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDT
NMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKA
LDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYE
NLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEV
RVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNT
ILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTA
YQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLV
EGFYPAPGLKTFLNMFVDSNQDARRRSXVNEDDNPPSPIGGDMMDSLISQ
LQPPPQQQPFPKQPGTSGAYPLTSPTSYHSTVNQSPSMMHTQSPGNLHAA
SSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMV
SPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTS
SQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAG
SYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCR
VALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLI
AFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIA
PPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQAD
IPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPP
GGRP