human ( Homo sapiens )
DDX5

Western result: +

GST GST-DDX5 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: DDX5
CBI Antibody Core Code: A0306
"Lot" Number: 2 Position: 100(479-578)
% Identical Homology of Target to Mouse Protein: 98% (NP_031866.1| DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 5; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
GSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGY
SSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSF
RTGNPTGTYQNGYDSTQQYG

Sequence Name: DDX5
Full name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
UniGene Number: N.A.
Accession Number: NM_004396
Source organism: human ( Homo sapiens )
Full length size (aa): 614
NCBI information page: click here

Full length sequence:
MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDE
LPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEAN
FPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLL
PAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTC
IYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEA
DRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHI
NIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVET
KRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDV
ASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNI
KQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAG
KRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFV
SAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAA
APMIGYPMPTGYSQ