human ( Homo sapiens )
CNOT1

Western result: +

GST GST-CNOT1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: CNOT1
CBI Antibody Core Code: A0732
"Lot" Number: 1 Position: 100(1508-1607)
% Identical Homology of Target to Mouse Protein: 100% (XP_486150.1| PREDICTED: RIKEN cDNA 6030411K04)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
QKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLT
YQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTND
LSQPTGFLAQPMKQAWATDD

Sequence Name: CNOT1
Full name:
UniGene Number: N.A.
Accession Number: AL832769; KIAA1007
Source organism: human ( Homo sapiens )
Full length size (aa): 2376
NCBI information page: click here

Full length sequence:
MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRC
LFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLH
YQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIK
QKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQ
EQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTM
MESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMART
HSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNP
SLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWK
HAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKS
LDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRH
ELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGE
QYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKW
LTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLAT
MLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSA
SSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSP
AKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVN
NDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANS
YFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYR
FFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKM
YYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRD
PPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAK
TVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIA
FIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYS
NFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLG
MITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSV
VFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPG
NLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTAT
VPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERA
VQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTA
GMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNC
ELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQA
ERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMK
QAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSR
NSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRA
YGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSM
ENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRG
NAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLRE
KAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFR
LCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHS
GEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLE
LNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIAR
MLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLL
VLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNL
KVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNL
QVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDI
FQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAI
QEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIE
KLFQSVAQCCMGQKQAQQVMEGTGAS