human ( Homo sapiens )
CP (Ceruloplasmin ferroxidase)

Western result: +

GST GST-hCP (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: hCP
CBI Antibody Core Code: A0011
"Lot" Number: 1 Position: To be posted
% Identical Homology of Target to Mouse Protein: 81% (NP_031778.1| ceruloplasmin)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
TAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTNRKE
RGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPI
GVRFNKNNEGTYYSPNYNPQ

Sequence Name: CP (Ceruloplasmin ferroxidase)
Full name: Ceruloplasmin (CP) ferroxidase
UniGene Number: N.A.
Accession Number: M13699
Source organism: human ( Homo sapiens )
Full length size (aa): 1046
NCBI information page: click here

Full length sequence:
KEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPDRIGRLYKK
ALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTF
HSHGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGE
GDGNCVTRIYHSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVV
MFSVVDENFSWYLEDNIKTYCSEPEKVDKDNEDFQESNRMYSVNGYTFGS
LPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFPA
TLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGK
HVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGS
YKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKG
AYPLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEW
TVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANG
RQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDEDFQESNK
MHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWRG
ERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCR
RQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLD
KGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDK
VKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGT
EDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALL
FLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNL
QGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDIF
PGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG