human ( Homo sapiens )
Np95 (UHRF1)

Western result: +

GST GST-Np95 (UHRF1) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Np95 (UHRF1)
CBI Antibody Core Code: A0181
"Lot" Number: 1 Position: 100(577-676)
% Identical Homology of Target to Mouse Protein: 49% (NP_035061.2| ubiquitin-like, containing PHD and RING finger domains, 1; nuclear protein 95)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
RYLLRRDDDEPGPWTKEGKDRIKKLGLTMQYPEGYLEALA
NREREKENSKREEEEQQEGGFASPRTGKGKWKRKSAGGGP
SRAGSPRRTSKKTKVEPYSL

Sequence Name: Np95 (UHRF1)
Full name: Nuclear zinc finger protein Np95
UniGene Number: N.A.
Accession Number: AF274048
Source organism: human ( Homo sapiens )
Full length size (aa): 793
NCBI information page: click here

Full length sequence:
MWIQVRTMDGRQTHTVDSLSRLTKVEELRRKIQELFHVEPGLQRLFYRGK
QMEDGHTLFDYEVRLNDTIQLLVRQSLVLPHSTKERDSELSDTDSGCCLG
QSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGA
WFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMN
SRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETR
TARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGP
SCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLS
SVPSEDEWYCPECRNDASEVVLAGERLRESKKKAKMASATSSSQRDWGKG
MACVGRTKECTIVPSNHYGPIPGIPVGTMWRFRVQVSESGVHRPHVAGIH
GRSNDGAYSLVLAGGYEDDVDHGNFFTYTGSGGRDLSGNKRTAEQSCDQK
LTNTNRALALNCFAPINDQEGAEAKDWRSGKPVRVVRNVKGGKNSKYAPA
EGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDDEPGPWTKEGKDRIKK
LGLTMQYPEGYLEALANREREKENSKREEEEQQEGGFASPRTGKGKWKRK
SAGGGPSRAGSPRRTSKKTKVEPYSLTAQQSSLIREDKSNAKLWNEVLAS
LKDRPASGSPFQLFLSKVEETFQCICCQELVFRPITTVCQHNVCKDCLDR
SFRAQVFSCPACRYDLGRSYAMQVNQPLQTVLNQLFPGYGNGR