human ( Homo sapiens )
BAZ1A

Western result: +

GST GST-BAZ1A (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: BAZ1A
CBI Antibody Core Code: A0173
"Lot" Number: 1 Position: 100(53-152)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PTALPPSEPKVDQKLPRSSERRGSGGGTQFPARSRAVAAG
EAAARGAAGPERGSPLGRRVSPRCLCSGEGGQVAVGVIAG
KRGRRGRDGSRRAPGGREMP

Sequence Name: BAZ1A
Full name: Bromodomain adjacent to zinc finger domain, 1A
UniGene Number: N.A.
Accession Number: NM_013448
Source organism: human ( Homo sapiens )
Full length size (aa): 1674
NCBI information page: click here

Full length sequence:
MEDASESSRGVAPLINNVVLPGSPLSLPVSVTGCKSHRVANKKVEARSEK
LLPTALPPSEPKVDQKLPRSSERRGSGGGTQFPARSRAVAAGEAAARGAA
GPERGSPLGRRVSPRCLCSGEGGQVAVGVIAGKRGRRGRDGSRRAPGGRE
MPLLHRKPFVRQKPPADLRPDEEVFYCKVTNEIFRHYDDFFERTILCNSL
VWSCAVTGRPGLTYQEALESEKKARQNLQSFPEPLIIPVLYLTSLTHRSR
LHEICDDIFAYVKDRYFVEETVEVIRNNGARLQCTILEVLPPSHQNGFAN
GHVNSVDGETIIISDSDDSETQSCSFQNGKKKDAIDPLLFKYKVQPTKKE
LHESAIVKATQISRRKHLFSRDKLKLFLKQHCEPQEGVIKIKASSLSTYK
IAEQDFSYFFPDDPPTFIFSPANRRRGRPPKRIHISQEDNVANKQTLASY
RSKATKERDKLLKQEEMKSLAFEKAKLKREKADALEAKKKEKEDKEKKRE
ELKKIVEEERLKKKEEKERLKVEREKEREKLREEKRKYVEYLKQWSKPRE
DMECDDLKELPEPTPVKTRLPPEIFGDALMVLEFLNAFGELFDLQDEFPD
GVTLEVLEEALVGNDSEGPLCELLFFFLTAIFQAIAEEEEEVAKEQLTDA
DTKGCSLKSLDLDSCTLSEILRLHILASGADVTSANAKYRYQKRGGFDAT
DDACMELRLSNPSLVKKLSSTSVYDLTPGEKMKILHALCGKLLTLVSTRD
FIEDYVDILRQAKQEFRELKAEQHRKEREEAAARIRKRKEEKLKEQEQKM
KEKQEKLKEDEQRNSTADISIGEEEREDFDTSIESKDTEQKELDQDMFTE
DEDDPGSHKRGRRGKRGQNGFKEFTRQEQINCVTRELLTADEEEALKQEH
QRKEKELLEKIQSAIACTNIFPLGRDRMYRRYWIFPSIPGLFIEEDYSGL
TEDMLLPRPSSFQNNVQSQDPQVSTKTGEPLMSESTSNIDQGPRDHSVQL
PKPVHKPNRWCFYSSCEQLDQLIEALNSRGHRESALKETLLQEKSRICAQ
LARFSEEKFHFSDKPQPDSKPTYSRGRSSNAYDPSQMCAEKQLELRLRDF
LLDIEDRIYQGTLGAIKVTDRHIWRSALESGRYELLSEENKENGIIKTVN
EDVEEMEIDEQTKVIVKDRLLGIKTETPSTVSTNASTPQSVSSVVHYLAM
ALFQIEQGIERRFLKAPLDASDSGRSYKTVLDRWRESLLSSASLSQVFLH
LSTLDRSVIWSKSILNARCKICRKKGDAENMVLCDGCDRGHHTYCVRPKL
KTVPEGDWFCPECRPKQRCRRLSFRQRPSLESDEDVEDSMGGEDDEVDGD
EEEGQSEEEEYEVEQDEDDSQEEEEVSLPKRGRPQVRLPVKTRGKLSSSF
SSRGQQQEPGRYPSRSQQSTPKTTVSSKTGRSLRKINSAPPTETKSLRIA
SRSTRHSHGPLQADVFVELLSPRRKRRGRKSANNTPENSPNFPNFRVIAT
KSSEQSRSVNIASKLSLQESESKRRCRKRQSPEPSPVTLGRRSSGRQGGV
HELSAFEQLVVELVRHDDSWPFLKLVSKIQVPDYYDIIKKPIALNIIREK
VNKCEYKLASEFIDDIELMFSNCFEYNPRNTSEAKAGTRLQAFFHIQAQK
LGLHVTPSNVDQVSTPPAAKKSRI