human ( Homo sapiens )
TRAP220

Western result: +

GST GST-TRAP220 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TRAP220
CBI Antibody Core Code: A0140
"Lot" Number: 1 Position: 100(799-898)
% Identical Homology of Target to Mouse Protein: 84% (NP_038662.1| Ppar binding protein isoform 1; TRAP 220)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
DCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDP
ADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGF
GEEYFDESSQSGDNDDFKGF

Sequence Name: TRAP220
Full name:
UniGene Number: N.A.
Accession Number: AF055994
Source organism: human ( Homo sapiens )
Full length size (aa): 1581
NCBI information page: click here

Full length sequence:
MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVM
SSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTE
CYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEF
SKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPL
DKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRS
LGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLP
ACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSK
DPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQH
PGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRF
SVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQR
CMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPLAGPGYGM
TTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQ
NPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLK
DNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKP
KHQTEDDFQRELFSMDVDSQKPIFDVNMTADTLDTPHITPAPSQCSTPPT
TYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDC
PAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPS
SDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFAS
QALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHH
SGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPS
NDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQ
TPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSH
SSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSG
GKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPG
GSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLG
SSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKK
PSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGE
FQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTSVAKIIISKHDGG
SPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSH
SKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKH
KKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNT
ILSADRPSRLSPDFMIGEEDDDLMDVALIGN