human ( Homo sapiens )
ATRX

Western result: +

GST GST-ATRX (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: ATRX
CBI Antibody Core Code: A0601
"Lot" Number: 2 Position: 100(701-800)
% Identical Homology of Target to Mouse Protein: 38% (NP_033556.1| alpha thalassemia/mental retardation syndrome X-linked homolog; X-linked nuclear protein; alpha thalassemia/mental retardation syndrome (X-linked))
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
KEVSRMSHSSSSDTDINEIHTNHKTLYDLKTQAGKDDKGK
RKRKSSTSGSDFDTKKGKSAKSSIISKKKRQTQSESSNYD
SELEKEIKSMSKIGAARTTK

Sequence Name: ATRX
Full name: putative DNA dependent ATPase and helicase (ATRX) mRNA, alternatively spliced product 3, complete cds
UniGene Number: N.A.
Accession Number: U72938.2, U72938
Source organism: human ( Homo sapiens )
Full length size (aa): 2454
NCBI information page: click here

Full length sequence:
MTAEPMSESKLNTLVQKLHDFLAHSSEESEETSSPPRLAMNQNTDKISGS
GSNSDMMENSKEEGTSSSEKSKSSGSSRSKRKPSIVTKYVESDDEKPLDD
ETVNEDASNENSENDITMQSLPKEDGLHGIVSCTACGQQVNHFQKDSIYR
HPSLQVLICKNCFKYYMSDDISRDSDGMDEQCRWCAEGGNLICCDFCHNA
FCKKCILRNLGRKELSTIMDENNQWYCYICHPEPLLDLVTACNSVFENLE
QLLQQNKKKIKVDSEKSNKVYEHTSRFSPKKTSSNCNGEEKKLDDSCSGS
VTYSYSALIVPKEMIKKAKKLIETTANMNSSYVKFLKQATDNSEISSATK
LRQLKAFKSVLADIKKAHLALEEDLNSEFRAMDAVNKEKNTKEHKVIDAK
FETKARKGEKPCALEKKDISKSEAKLSRKQVDSEHMHQNVPTEEQRTNKS
TGGEHKKSDRKEEPQYEPANTSEDLDMDIVSVPSSVPEDIFENLETAMEV
QSSVDHQGDGSSGTEQEVESSSVKLNISSKDNRGGIKSKTTAKVTKELYV
KLTPVSLSNSPIKGADCQEVPQDKDGYKSCGLNPKLEKCGLGQENSDNEH
LVENEVSLLLEESDLRRSPRVKTTPLRRPTETNPVTSNSDEECNETVKEK
QKLSVPVRKKDKRNSSDSAIDNPKPNKLPKSKQSETVDQNSDSDEMLAIL
KEVSRMSHSSSSDTDINEIHTNHKTLYDLKTQAGKDDKGKRKRKSSTSGS
DFDTKKGKSAKSSIISKKKRQTQSESSNYDSELEKEIKSMSKIGAARTTK
KRIPNTKDFDSSEDEKHSKKGMDNQGHKNLKTSQEGSSDDAERKQERETF
SSAEGTVDKDTTIMELRDRLPKKQQASASTDGVDKLSGKEQSFTSLEVRK
VAETKEKSKHLKTKTCKKVQDGLSDIAEKFLKKDQSDETSEDDKKQSKKG
TEEKKKPSDFKKKVIKMEQQYESSSDGTEKLPEREEICHFPKGIKQIKNG
TTDGEKKSKKIRDKTSKKKDELSDYAEKSTGKGDSCDSSEDKKSKNGAYG
REKKRCKLLGKSSRKRQDCSSSDTEKYSMKEDGCNSSDKRLKRIELRERR
NLSSKRNTKEIQSGSSSSDAEESSEDNKKKKQRTSSKKKAVIVKEKKRNS
LRTSTKRKQADITSSSSSDIEDDDQNSIGEGSSDEQKIKPVTENLVLSSH
TGFCQSSGDEALSKSVPVTVDDDDDDNDPENRIAKKMLLEEIKANLSSDE
DGSSDDEPEEGKKRTGKQNEENPGDEEAKNQVNSESDSDSEESKKPRYRH
RLLRHKLTVSDGESGEEKKTKPKEHKEVKGRNRRKVSSEDSEDSDFQESG
VSEEVSESEDEQRPRTRSAKKAELEENQRSYKQKKKRRRIKVQEDSSSEN
KSNSEEEEEEKEEEEEEEEEEEEEEEDENDDSKSPGKGRKKIRKILKDDK
LRTETQNALKEEEERRKRIAEREREREKLREVIEIEDASPTKCPITTKLV
LDEDEETKEPLVQVHRNMVIKLKPHQVDGVQFMWDCCCESVKKTKKSPGS
GCILAHCMGLGKTLQVVSFLHTVLLCDKLDFSTALVVCPLNTALNWMNEF
EKWQEGLKDDEKLEVSELATVKRPQERSYMLQRWQEDGGVMIIGYEMYRN
LAQGRNVKSRKLKEIFNKALVDPGPDFVVCDEGHILKNEASAVSKAMNSI
RSRRRIILTGTPLQNNLIEYHCMVNFIKENLLGSIKEFRNRFINPIQNGQ
CADSTMVDVRVMKKRAHILYEMLAGCVQRKDYTALTKFLPPKHEYVLAVR
MTSIQCKLYQYYLDHLTGVGNNSEGGRGKAGAKLFQDFQMLSRIWTHPWC
LQLDYISKENKGYFDEDSMDEFIASDSDETSMSLSSDDYTKKKKKGKKGK
KDSSSSGSGSDNDVEVIKVWNSRSRGGGEGNVDETGNNPSVSLKLEESKA
TSSSNPSSPAPDWYKDFVTDADAEVLEHSGKMVLLFEILRMAEEIGDKVL
VFSQSLISLDLIEDFLELASREKTEDKDKPLIYKGEGKWLRNIDYYRLDG
STTAQSRKKWAEEFNDETNVRGRLFIISTKAGSLGINLVAANRVIIFDAS
WNPSYDIQSIFRVYRFGQTKPVYVYRFLAQGTMEDKIYDRQVTKQSLSFR
VVDQQQVERHFTMNELTELYTFEPDLLDDPNSEKKKKRDTPMLPKDTILA
ELLQIHKEHIVGYHEHDSLLDHKEEEELTEEERKAAWAEYEAEKKGLTMR
FNIPTGTNLPPVSFNSQTPYIPFNLGALSAMSNQQLEDLINQGREKVVEA
TNSVTAVRIQPLEDIISAVWKENMNLSEAQVQALALSRQASQELDVKRRE
AIYNDVLTKQQMLISCVQRILMNRRLQQQYNQQQQQQMTYQQATLGHLMM
PKPPNLIMNPSNYQQIDMRGMYQPVAGGMQPPPLQRAPPPMRSKNPGPSQ
GKSM