human ( Homo sapiens )
TF-IRF7D

Western result: +

control TF-IRF7D (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: TF-IRF7D
CBI Antibody Core Code: A0119
"Lot" Number: 1 Position: 20(1-20)
% Identical Homology of Target to Mouse Protein: 61% (NP_032538.1| low density lipoprotein receptor-related protein 1; alpha 2-macroglobulin receptor)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
MPVPERPAAGPDSPRPGTRR

Sequence Name: TF-IRF7D
Full name:
UniGene Number: N.A.
Accession Number: NM_004031
Source organism: human ( Homo sapiens )
Full length size (aa): 516
NCBI information page: click here

Full length sequence:
MPVPERPAAGPDSPRPGTRRAAPRVLFGEWLLGEISSGCYEGLQWLDEAR
TCFRVPWKHFARKDLSEADARIFKAWAVARGRWPPSSRGGGPPPEAETAE
RAGWKTNFRCALRSTRRFVMLRDNSGDPADPHKVYALSRELCWREGPGTD
QTEAEAPAAVPPPQGGPPGPFLAHTHAGLQAPGPLPAPAGDEGDLLLQAV
QQSCLADHLLTASWGADPVPTKAPGEGQEGLPLTGACAGGPGLPAGELYG
WAVETTPSPGPQPAALTTGEAAAPESPHQAEPYLSPSPSACTAVQEPSPG
ALDVTIMYKGRTVLQKVVGHPSCTFLYGPPDPAVRATDPQQVAFPSPAEL
PDQKQLRYTEELLRHVAPGLHLELRGPQLWARRMGKCKVYWEVGGPPGSA
SPSTPACLLPRNCDTPIFDFRVFFRELVEFRARQRRGSPRYTIYLGFGQD
LSAGRPKEKSLVLVKLEPWLCRVHLEGTQREGVSSLDSSSLSLCLSSANS
LYDDIECFLMELEQPA