human ( Homo sapiens )
SVCT2 (or SLC23A2)

Western result: +

control SVCT2 (or SLC23A2) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: SVCT2 (or SLC23A2)
CBI Antibody Core Code: A0425
"Lot" Number: 1 Position: 20(630-649)
% Identical Homology of Target to Mouse Protein: 80% (NP_061294.2| solute carrier family 23 (nucleobase transporters), member 2; sodium-dependent vitamin C transporter; solute carrier family 23 (nucleobase transporters), member 1)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
WKGLRKSDNSRSSDEDSQAT

Sequence Name: SVCT2 (or SLC23A2)
Full name: solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2)
UniGene Number: N.A.
Accession Number: NM_005116
Source organism: human ( Homo sapiens )
Full length size (aa): 650
NCBI information page: click here

Full length sequence:
MMGIGKNTTSKSMEAGSSTEGKYEDEAKHRAFFTLPVVINGGATSSGEQD
NEDTELMAIYTTENGIAEKSSLAETLDSTGSLDPQRSDMIYTIEDVPPWY
LCIFLGLQHYLTCFSGTIAVPFLLADAMCVGYDQWATSQLTGTIFFCVGI
TTLLQTTFGCRLPLFQASAFAFLAPARAILSSDKWKCNTTDVSVANGTAE
LLHTEHIWYPRIREVQGAIIMSSLIEVVIGLLGLPGALLKYIGPLTITPT
VALIGLSGFQAAGERAGKHWGIAMLTIFLVLLFSQYARNVKFPLPIYKSK
KGWTAYKLQLFKMFPIILAILVSWLLCFIFTVTDVFPPDSTKYGFYARTD
ARQGVLLVAPWFKVPYPFQWGLPTVSAAGVIGMLSAVVASIIESIGDYYA
CARLSCAPPPPIHAINRGIFVEGLSCVLDGIFGTGNGSTSSSPNIGVLGI
TKVGSRRVIQCGAALMLALGMIGKFSALFASLPDPVLGALFCTLFGMITA
VGLSNLQFIDLNSSRNLFVLGFSIFFGLVLPSYLRQNPLVTGITGIDQVL
NVLLTTAMFVGGCVAFILDNTIPGTPEERGIRKWKKGVGKGNKSLDGMES
YNLPFGMNIIKKYRCFSYLPISPTFVGYTWKGLRKSDNSRSSDEDSQATG