human ( Homo sapiens )
S5

Western result: +

GST GST-S5 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: S5
CBI Antibody Core Code: A0441
"Lot" Number: 1 Position: 100(321-420)
% Identical Homology of Target to Mouse Protein: 85% (NP_542121.1| proteasome 26S non-ATPase subunit 5)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
ILGSNVEGKQVLQKTGTRFERLLMRIGHQSKNAPVELKIR
CLDAISSLLYLPPEQQTDDLLRMTESWFSSLSRDPLELFR
GISSQPFPELHCAALKVFTA

Sequence Name: S5
Full name: proteasome 26S non-ATPase subunit 5; 26S protease subunit S5 basic; 26S proteasome subunit S5B; 26S protease subunit 5b=50 kda subunit; 26S proteasome non-ATPase regulatory subunit 5
UniGene Number: N.A.
Accession Number: NP_005038
Source organism: human ( Homo sapiens )
Full length size (aa): 504
NCBI information page: click here

Full length sequence:
MAAQALALLREVARLEAPLEELRALHSVLQAVPLNELRQQAAELRLGPLF
SLLNENHREKTTLCVSILERLLQAMEPVHVARNLRVDLQRGLIHPDDSVK
ILTLSQIGRIVENSDAVTEILNNAELLKQIVYCIGGENLSVAKAAIKSLS
RISLTQAGLEALFESNLLDDLKSVMKTNDIVRYRVYELIIEISSVSPESL
NYCTTSGLVTQLLRELTGEDVLVRATCIEMVTSLAYTHHGRQYLAQEGVI
DQISNIIVGADSDPFSSFYLPGFVKFFGNLAVMDSPQQICERYPIFVEKV
FEMIESQDPTMIGVAVDTVGILGSNVEGKQVLQKTGTRFERLLMRIGHQS
KNAPVELKIRCLDAISSLLYLPPEQQTDDLLRMTESWFSSLSRDPLELFR
GISSQPFPELHCAALKVFTAIANQPWAQKLMFNSPGFVEYVVDRSVEHDK
ASKDAKYELVKALANSKTIAEIFGNPNYLRLRTYLSEGPYYVKPVSTTAV
EGAE