human ( Homo sapiens )
S10b

Western result: +

GST GST-S10b (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: S10b
CBI Antibody Core Code: A0437
"Lot" Number: 1 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 85% (NP_080235.2| proteasome 26S ATPase subunit 6)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQY
EKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYV
VGCRRQLDKSKLKPGTRVAL

Sequence Name: S10b
Full name: 26S protease regulatory subunit S10B; proteasome 26S ATPase subunit 6; conserved ATPase domain protein 44; proteasome subunit p42
UniGene Number: N.A.
Accession Number: NP_002797
Source organism: human ( Homo sapiens )
Full length size (aa): 389
NCBI information page: click here

Full length sequence:
MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKAL
QSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVAL
DMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIE
LPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVS
SSIVDKYIGESARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSAD
REIQRTLMELLNQMDGFDTLHRVKMIMATNRPDTLDPALLRPGRLDRKIH
IDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEA
GMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV