human ( Homo sapiens )
pygo2

Western result: +-

GST GST-pygo2 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: pygo2
CBI Antibody Core Code: A0705
"Lot" Number: 1 Position: 100(75-174)
% Identical Homology of Target to Mouse Protein: 95% (XP_130932.2| pygopus 2)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
SNPFEDDFGAPKVGVAAPPFLGSPVPFGGFRVQGGMAGQV
PPGYSTGGGGGPQPLRRQPPPFPPNPMGPAFNMPPQGPGY
PPPGNMNFPSQPFNQPLGQN

Sequence Name: pygo2
Full name: pygopus 2
UniGene Number: N.A.
Accession Number: NP_612157
Source organism: human ( Homo sapiens )
Full length size (aa): 406
NCBI information page: click here

Full length sequence:
MAASAPPPPDKLEGGGGPAPPPAPPSTGRKQGKAGLQMKSPEKKRRKSNT
QGPAYSHLTEFAPPPTPMVDHLVASNPFEDDFGAPKVGVAAPPFLGSPVP
FGGFRVQGGMAGQVPPGYSTGGGGGPQPLRRQPPPFPPNPMGPAFNMPPQ
GPGYPPPGNMNFPSQPFNQPLGQNFSPPSGQMMPGPVGGFGPMISPTMGQ
PPRAELGPPSLSQRFAQPGAPFGPSPLQRPGQGLPSLPPNTSPFPGPDPG
FPGPGGEDGGKPLNPPASTAFPQEPHSGSPAAAVNGNQPSFPPNSSGRGG
GTPDANSLAPPGKAGGGSGPQPPPGLVYPCGACRSEVNDDQDAILCEASC
QKWFHRECTGMTESAYGLLTTEASAVWACDLCLKTKEIQSVYIREGMGQL
VAANDG