human ( Homo sapiens )
Prostate specific antigen (PSA)

Western result: +

GST GST-PSA (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PSA
CBI Antibody Core Code: A0080
"Lot" Number: 1 Position: 100(123-222)
% Identical Homology of Target to Mouse Protein: 60% (NP_034770.1| kallikrein 11)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCA
QVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQG
ITSWGSEPCALPERPSLYTK

Sequence Name: Prostate specific antigen (PSA)
Full name: Prostate specific antigen (PSA)
UniGene Number: N.A.
Accession Number: M26663
Source organism: human ( Homo sapiens )
Full length size (aa): 238
NCBI information page: click here

Full length sequence:
RIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVI
LLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLML
LRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQC
VDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVL
QGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP