human ( Homo sapiens )
PMCA1 (ATP2B1)

Western result: +

GST GST-PMCA1 (ATP2B1) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PMCA1 (ATP2B1)
CBI Antibody Core Code: A0236
"Lot" Number: 2 Position: 100(1121-1220)
% Identical Homology of Target to Mouse Protein: 86% (XP_357338.1| similar to plasma membrane calcium pump isoform 1b; PMCA1b)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
VNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIP
LIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEM
NKSATSSSPGSPLHSLETSL

Sequence Name: PMCA1 (ATP2B1)
Full name: PMCA1 (ATP2B1) or (ATP2B1) Plasma membrane 1 Ca2++ ATPase isoform 2b
UniGene Number: N.A.
Accession Number: NM_001682
Source organism: human ( Homo sapiens )
Full length size (aa): 1220
NCBI information page: click here

Full length sequence:
MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQ
ESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQ
LVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGE
TGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGG
QVIQIPVADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHV
KKSLDKDPLLLSGTHVREGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEK
KDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKK
KANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQ
KRPWLAECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKM
MKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKV
PEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALL
GLLLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFS
KGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICL
AFRDFPAGEPEPEWDNENDIVTGLTCIAVVGIEDPVRPEVPDAIKKCQRA
GITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEI
EQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSDQRQVVAVTGDGTN
DGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYD
SISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASL
ALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAG
EKFFDIDSGRNAPLHAPPSEHYTIVFNTFVLMQLFNEINARKIHGERNVF
EGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMG
TLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAEREL
RRGQILWFRGLNRIQTQIRVVNAFRSSLYEGLEKPESRSSIHNFMTHPEF
RIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEM
NKSATSSSPGSPLHSLETSL