human ( Homo sapiens )
MetAP2

Antibody Name/Abbreviation: MetAP2
CBI Antibody Core Code: A0194
"Lot" Number: 2 Position: 100(51-150)
% Identical Homology of Target to Mouse Protein: 61% (NP_062622.1| methionine aminopeptidase 2; eIF-2-associated p67; initiation factor 2-associated protein (p67)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
AGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDG
DGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFP
KGQECEYPPTQDGRTAAWRT

Other western:
Code: A0194
Lot: 1

Western result: ++

GST GST-MetAP2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: MetAP2
Full name: methionyl aminopeptidase 2; methionine aminopeptidase; eIF-2-associated p67
UniGene Number: N.A.
Accession Number: NP_006829
Source organism: human ( Homo sapiens )
Full length size (aa): 478
NCBI information page: click here

Full length sequence:
MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSA
AGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKK
KKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRT
TSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKL
EDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKI
DFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDV
GEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGG
EATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTK
HLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDI
KGSYTAQFEHTILLRPTCKEVVSRGDDY