human ( Homo sapiens )
MEF2D

Western result: +++

GST GST-MEF2D (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: MEF2D
CBI Antibody Core Code: A0198
"Lot" Number: 1 Position: 100(11-110)
% Identical Homology of Target to Mouse Protein: 100% (NP_598426.1| myocyte enhancer factor 2D)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
ITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNH
SNKLFQYASTDMDKVLLKYTEYNEPHESRTNADIIETLRK
KGFNGCDSPEPDGEDSLEQS

Sequence Name: MEF2D
Full name: Myocyte-specific enhancer factor 2D
UniGene Number: N.A.
Accession Number: Q14814
Source organism: human ( Homo sapiens )
Full length size (aa): 521
NCBI information page: click here

Full length sequence:
MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNH
SNKLFQYASTDMDKVLLKYTEYNEPHESRTNADIIETLRKKGFNGCDSPE
PDGEDSLEQSPLLEDKYRRASEELDGLFRRYGSTVPAPNFAMPVTVPVSN
QSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPA
SAGAMLGGDLNSANGACPSPVGNGYVSARASPGLLPVANGNSLNKVIPAK
SPPPPTHSTQLGAPSRKPDLRVITSQAGKGLMHHLTEDHLDLNNAQRLGV
SQSTHSLTTPVVSVATPSLLSQGLPFSSMPTAYNTDYQLTSAELSSLPAF
SSPGGLSLGNVTAWQQPQQPQQPQQPQPPQQQPPQPQQPQPQQPQQPQQP
PQQQSHLVPVSLSNLIPGSPLPHVGAALTVTTHPHISIKSEPVSPSRERS
PAPPPPAVFPAARPEPGDGLSSPAGGSYETGDRDDGRGDFGPTLGLLRPA
PEPEAEGSAVKRMRLDTWTLK