human ( Homo sapiens )
LRPPRC

Western result: +

GST GST-LRPPRC (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: LRPPRC
CBI Antibody Core Code: A0682
"Lot" Number: 1 Position: 100(897-996)
% Identical Homology of Target to Mouse Protein: 87% (NP_082509.1| leucine-rich PPR motif-containing protein; leucine rich protein LRP130)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
AFLQTGNYKEAKKIIETPGIRARSARLQWFCDRCVANNQV
ETLEKLVELTQKLFECDRDQMYYNLLKLYKINGDWQRADA
VWNKIQEENVIPREKTLRLL

Sequence Name: LRPPRC
Full name: leucine-rich PPR motif-containing protein
UniGene Number: N.A.
Accession Number: NP_573566
Source organism: human ( Homo sapiens )
Full length size (aa): 1394
NCBI information page: click here

Full length sequence:
MAALLRSARWLLRAGAAPRLPLSLRLLPGGPGRLHAASYLPAARAGPVAG
GLLSPARLYAIAAKEKDIQEESTFSSRKISNQFDWALMRLDLSVRRTGRI
PKKLLQKVFNDTCRSGGLGGSHALLLLRSCGSLLPELKLEERTEFAHRIW
DTLQKLGAVYDVSHYNALLKVYLQNEYKFSPTDFLAKMEEANIQPNRVTY
QRLIASYCNVGDIEGASKILGFMKTKDLPVTEAVFSALVTGHARAGDMEN
AENILTVMRDAGIEPGPDTYLALLNAYAEKGDIDHVKQTLEKVEKSELHL
MDRDLLQIIFSFSKAGYPQYVSEILEKVTCERRYIPDAMNLILLLVTEKL
EDVALQILLACPVSKEDGPSVFGSFFLQHCVTMNTPVEKLTDYCKKLKEV
QMHSFPLQFTLHCALLANKTDLAKALMKAVKEEGFPIRPHYFWPLLVGRR
KEKNVQGIIEILKGMQELGVHPDQETYTDYVIPCFDSVNSARAILQENGC
LSDSDMFSQAGLRSEAANGNLDFVLSFLKSNTLPISLQSIRSSLLLGFRR
SMNINLWSEITELLYKDGRYCQEPRGPTEAVGYFLYNLIDSMSDSEVQAK
EEHLRQYFHQLEKMNVKIPENIYRGIRNLLESYHVPELIKDAHLLVESKN
LDFQKTVQLTSSELESTLETLKAENQPIRDVLKQLILVLCSEENMQKALE
LKAKYESDMVTGGYAALINLCCRHDKVEDALNLKEEFDRLDSSAVLDTGK
YVGLVRVLAKHGKLQDAINILKEMKEKDVLIKDTTALSFFHMLNGAALRG
EIETVKQLHEAIVTLGLAEPSTNISFPLVTVHLEKGDLSTALEVAIDCYE
KYKVLPRIHDVLCKLVEKGETDLIQKAMDFVSQEQGEMVMLYDLFFAFLQ
TGNYKEAKKIIETPGIRARSARLQWFCDRCVANNQVETLEKLVELTQKLF
ECDRDQMYYNLLKLYKINGDWQRADAVWNKIQEENVIPREKTLRLLAEIL
REGNQEVPFDVPELWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKG
AYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGF
TLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALA
MKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENI
ENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAI
YKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGK
ASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTK
LDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS