human ( Homo sapiens )
KIAA1335

Western result: +

GST GST-KIAA1335 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: KIAA1335
CBI Antibody Core Code: A0703
"Lot" Number: 1 Position: 100(1302-1401)
% Identical Homology of Target to Mouse Protein: 63% (XP_485094.1| RIKEN cDNA 5430439G14)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
AMEGEPTAIPSQPFKVKHELLKEPWKESAEGQNVFPTYPL
EGSELKSEDMDFENKDDYDRDGNCHSQDYPGKYSEEESKS
STSGITGDIGDELQEARAPT

Sequence Name: KIAA1335
Full name: KIAA1335 protein
UniGene Number: N.A.
Accession Number: BAA92573
Source organism: human ( Homo sapiens )
Full length size (aa): 2041
NCBI information page: click here

Full length sequence:
LKPMMLRRLKDDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTK
GANQHNMPNLINTMMELRKCCNHPYLINGAEEKILEDFRKTHSPDAPDFQ
LQAMIQAAGKLVLIDKLLPKLIAGGHKVLIFSQMVRCLDILEDYLIQRRY
TYERIDGRVRGNLRQAAIDRFCKPDSDRFVFLLCTRAGGLGINLTAADTC
IIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLK
LGLDKAVLQDINRKGGTNGVQQLSKMEVEDLLRKGAYGALMDEEDEGSKF
CEEDIDQILQRRTHTITIQSEGKGSTFAKASFVASGNRTDISLDDPNFWQ
KWAKIAELDTEAKNEKESLVIDRPRVRKQTKHYNSFEEDELMEFSELDSD
SDERPTRSRRLNDKARRYLRAECFRVEKNLLIFGWGRWKDILTHGRFKWH
LNEKDMEMICRALLVYCVKHYKGDEKIKSFIWELITPTKDGQAQTLQNHS
GLSAPVPRGRKGKKTKNQLLIPELKDADWLATCNPEVVLHDDGYKKHLKQ
HCNKVLLRVRMLYYLKAEILGEAAEKAFEGSPARELDVPLPDIDYMEIPV
DWWDAEADKSLLIGVFKHGYERYNAMRADPALCFLEKVGMPDEKSLSAEQ
GVTDGTSDIPERGNTDKEDNAEDKVDGLQKQTESSSDGGDGVFSEKKDDS
RAAQDGSDPDKSPWPVSSALTARLRRLVTVYQRCNRKELCRPEILGPGNQ
GYWVQEEMFRRTSEMDLINKEAQKRWTRREQADFYRTVSSFGVVYDQEKK
TFDWTQFRIISRLDKKSDESLEQYFYSFVAMCRNVCRLPTWKDGGPPDTT
IYVEPITEERAARTLYRIELLRKVREQVLKCPQLHERLQLCRPSLYLPVW
WECGKHDRDLLIGTAKHGLNRTDCYIMNDPQLSFLDAYRNYAQHKRSGTQ
APGNLCCLYQTNSKLYESLTYSQMSRTSESLENEPENLVRVESRDDHLSL
PDVTCENFISKVQDVISINHDESLLPESLESMMYGKKVLSQEPSSFQESP
STNTESRKDVITISISKDGNCQSGGPEAEIASGPTFMGSLEAGGVAQANI
KNGKHLLMSISKEGELCCSEAGQRPENIGQLEAKCLASPSLNPGNESGFV
DMCSLSVCDSKRNLSSDQQLIDLLENKSLESKLILSQNHSDEEEEEEENE
EENLAMAVGMGERPEVLHLTEPTTNISREKNQGFQDETKKGSLEVANQTP
GLQRAFPAPAACQCHCKHMERWMHGLENDEFEIEKPKAYIPDLFKSKTNT
IAMEGEPTAIPSQPFKVKHELLKEPWKESAEGQNVFPTYPLEGSELKSED
MDFENKDDYDRDGNCHSQDYPGKYSEEESKSSTSGITGDIGDELQEARAP
TIAQLLQEKTLYSFSEWPKDRVIINRLDNICHVVLKGKWPSSQQYEPSGT
LPTPVLTSSAGSRTSLSEPEAAEHSFSNGAALAAQIHKESFLAPVFTKDE
QKHRRPYEFEVERDAKARGLEQFSATHGHTPIILNGWHGESAMDLSCSSE
GSPGATSPFPVSASTPKIGAISSLQGALGMDLSGILQAGLIHPVTGQIVN
GSLRRDDAATRRRRGRRKHVEGGMDLIFLKEQTLQAGILEVHEDPGQATL
STTHPEGPGPATSAPEPATAASSQAEKSIPSKSLLDWLRQQADYSLEVPG
FGANFSDKPKQRRPRCKEPGKLDVSSLSGEERVPAIPKEPGLRGFLPENK
FNHTLAEPILRDTGPRRRGRRPRSELLKAPSIVADSPSGMGPLFMNGLIA
GMDLVGLQNMRNMPGIPLTGLVGFPAGFATMPTGEEVKSTLSMLPMMLPG
MAAVPQMFGVGGLLSPPMATTCTSTAPASLSSTTKSGTAVTEKTAEDKPS
SHDVKTDTLAEDKPGPGPFSDQSEPAITTSSPVAFNPFLIPGVSPGLIYP
SMFLSPGMGMALPAMQQARHSEIVGLESQKRKKKKTKGDNPNSHPEPAPS
CEREPSGDENCAEPSAPLPAEREHGAQAGEGALKDSNNDTN