human ( Homo sapiens )
IVa2

Western result: +

GST GST-IVa2 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: IVa2
CBI Antibody Core Code: A0782
"Lot" Number: 3 Position: 100(5-104)
% Identical Homology of Target to Mouse Protein: 31% (NP_067370.2| hypoxia up-regulated 1; 170 kDa glucose regulated protein GRP170 precursor; calcium binding protein, 140 kDa; 150-kDa oxygen regulated protein; 140 kDa; calcium binding protein 140)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
GRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADEDPA
PVERHDPGPSGRAPTTAVQRKPPQPAKRGDMLDRDAVEQV
TELWDRLELLGQTLKSMPTA

Sequence Name: IVa2
Full name: unnamed protein product
UniGene Number: N.A.
Accession Number: CAB40667
Source organism: human ( Homo sapiens )
Full length size (aa): 449
NCBI information page: click here

Full length sequence:
METRGRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADEDPAPVERHD
PGPSGRAPTTAVQRKPPQPAKRGDMLDRDAVEQVTELWDRLELLGQTLKS
MPTADGLKPLKNFASLQELLSLGGERLLADLVRENMRVRDMLNEVAPLLR
DDGSCSSLNYQLHPVIGVIYGPTGCGKSQLLRNLLSSQLISPTPETVFFI
APQVDMIPPSELKAWEMQICEGNYAPGPDGTIIPQSGTLRPRFVKMAYDD
LILEHNYDVSDPRNIFAQAAARGPIAIIMDECMENLGGHKGVSKFFHAFP
SKLHDKFPKCTGYTVLVVLHNMNPRRDMAGNIANLKIQSKMHLISPRMHP
SQLNRFVNTYTKGLPLAISLLLKDIFRHHAQRSCYDWIIYNTTPQHEALQ
WCYLHPRDGLMPMYLNIQSHLYHVLEKIHRTLNDRDRWSRAYRARKTPK