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human ( Homo sapiens )
HnRNP C1/C2


Antibody Name/Abbreviation: HnRNP C1/C2
CBI Antibody Core Code: A0567

"Lot" Number: 2 Position: 100(206-307)
% Identical Homology of Target to Mouse Protein: 37% (NP_058580.1| heterogeneous nuclear ribonucleoprotein C; heterogeneous nuclear ribonucleoprotein C1; heterogeneous nuclear ribonucleoprotein C2; hnRNP C1; hnRNP C2)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
VDSLLENLEKMEKEQSKQAVEMKNDKSEEEQSSSSVKKDE
TNVKMESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDD
EKEAEEGEDDRDSANGEDDS


Other western:
Code: A0567
Lot: 1


Western result: ++
GST
GST-HnRNP C1/C2
(fragment)

N.A. kDa (recombinant)
The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.




Sequence Name: HnRNP C1/C2
Full name: Heterogeneous nuclear ribonucleoproteins C1/C2
UniGene Number: N.A.
Accession Number: P07910
Source organism: human ( Homo sapiens )
Full length size (aa): 306
NCBI information page: click here

Full length sequence:
MASNVTNKTDPRSMNSRVFIGNLNTLVVKKSDVEAIFSKYGKIVGCSVHK
GFAFVQYVNERNARAAVAGEDGRMIAGQVLDINLAAEPKVNRGKAGVKRS
AAEMYGSVTEHPSPSPLLSSSFDLDYDFQRDYYDRMYSYPARVPPPPPIA
RAVVPSKRQRVSGNTSRRGKSGFNSKSGQRGSSKSGKLKGDDLQAIKKEL
TQIKQKVDSLLENLEKMEKEQSKQAVEMKNDKSEEEQSSSSVKKDETNVK
MESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDDEKEAEEGEDDRDSA
NGEDDS





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© Monday, February 16, 2009 by the CBI Antibody Core and the Center for Biomedical Inventions, University of Texas Southwestern Medical School.