human ( Homo sapiens )
Astrin

Western result: +

GST GST-Astrin (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Astrin
CBI Antibody Core Code: A1201
"Lot" Number: 1 Position: 100 (407-506)
% Identical Homology of Target to Mouse Protein: 60% (NP_059103.1| sperm associated antigen 5; astrin; microtubule-associated protein; s17 protein)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
GTKHSTSETEQLLCGRPPDLTALSRHDLEDNLLSSLVIVE
FLSRQLRDWKSQLAVPHPETQDSSTQTDTSHSGITNKLQH
LKESHEMGQALQQARNVMQS

Sequence Name: Astrin
Full name:
UniGene Number: N.A.
Accession Number: AF399910
Source organism: human ( Homo sapiens )
Full length size (aa): 1193
NCBI information page:

Full length sequence:
MWRVKKLSLSLSPSPQTGKPSMRTPLRELTLQPGALTTSGKRSPACSSLT
PSLCKLGLQEGSNNSSPVDFVNNKRTDLSSEHFSHSSKWLETCQHESDEQ
PLDPIPQISSTPKTSEEAVDPLGNYMVKTIVLVPSPLGQQQDMIFEARLD
TMAETNSISLNGPLRTDDLVREEVAPCMGDRFSEVAAVSEKPIFQESPSH
LLEESPPNPCSEQLHCSKESLSSRTEAVREDLVPSESNAFLPSSVLWLSP
STALAADFRVNHVDPEEEIVEHGAMEEREMRFPTHPKESETEDQALVSSV
EDILSTCLTPNLVEMESQEAPGPAVEDVGRILGSDTESWMSPLAWLEKGV
NTSVMLENLRQSLSLPSMLRDAAIGTTPFSTCSVGTWFTPSAPQEKSTNT
SQTGLVGTKHSTSETEQLLCGRPPDLTALSRHDLEDNLLSSLVIVEFLSR
QLRDWKSQLAVPHPETQDSSTQTDTSHSGITNKLQHLKESHEMGQALQQA
RNVMQSWVLISKELISLLHLSLLHLEEDKTTVNQESRRAETLVCCCFDLL
KKLRAKLQSLKAEREEARHREEMALRGKDAAEIVLEAFCAHASQRISQLE
QDLASMREFRGLLKDAQTQLVGLHAKQEELVQQTVSLTSTLQQDWRSMQL
DYTTWTALLSRSRQLTEKLTVKSQQALQERDVAIEEKQEVSRVLEQVSAQ
LEECKGQTEQLELENIRLATDLRAQLQILANMDSQLKELQSQHTHCAQDL
AMKDELLCQLTQSNEEQAAQCVKEEMALKHMQAELQQQQAVLAKEVRDLK
ETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCENLKDTVENLTA
KLASTIADNQEQDLEKTRQYSQKLGLLTEQLQSLTLFLQTKLKEKTEQET
LLLSTACPPTQEHPLPNDRTFLGSILTAVADEEPESTPVPLLGSDKSAFT
RVASMVSLQPAETPGMEESLAEMSIMTTELQSLCSLLQESKEEAIRTLQR
KICELQARLQAQEEQHQEVQKAKEADIEKLNQALCLRYKNEKELQEVIQQ
QNEKILEQIDKSGELISLREEVTHLTRSLRRAETETKVLQEALAGQLDSN
CQPMATNWIQEKVWLSQEVDKLRVMFLEMKNEKEKLMIKFQSHRNILEEN
LRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLEFLS